Abstract
Flexibacter FS-1 filaments are unable to glide on agarose gels or on charge-neutralized glass unless those substrata are supplemented with the charged, cold water-soluble fraction of agar, or a variety of polyanionic polysaccharides derived from plants, yeasts and bacteria. Two graft polymers of xanthan gum also promote gliding motility under these conditions, as do an extracellular product of the bacteria themselves. A number of other polymers and small molecular species are ineffective as supplements. These results are considered in the context of the soil habitat of this Flexibacter. Among a variety of other gliding bacteria tested, several strains of the order Cytophagales also were unable to glide on agarose.
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Abbreviations
- CWSF:
-
Cold water-soluble fraction of agar
- PMC:
-
5 mM K phosphate buffer (pH 7)+0.1 mM MgCl2+0.5 mM CaCl2
References
Adams GA (1965) Complete acid hydrolysis. In: Whistler RL, BeMiller JN (eds) Methods in carbohydrate chemistry, vol V. Academic Press, New York, pp 269–276
Arlauskas J, Burchard RP (1979) Substrate specificity for gliding motility of Flexibacter FS-1. In: Abstracts of the 79th annual meeting of the American Society for Microbiology, Washington, DC, p 99
Burchard RP (1980) Gliding motility of bacteria. BioScience 30:157–162
Burchard RP (1981) Gliding motility of prokaryotes: Ultrastructure, physiology, and genetics. Ann Rev Microbiol 35:497–529
Burchard RP (1982a) Evidence for contractile flexing of the gliding bacterium Flexibacter FS-1. Nature 298:663–665
Burchard RP (1982b) Trail following by gliding bacteria. J Bacteriol 152:495–501
Castenholz R (1973) Movements. In: Carr NG, Whitton BA (eds) The biology of the blue-green algae. Univ. of California Press, Berkeley, pp 320–339
Costenbader CJ, Burchard RP (1978) Effect of cell length on gliding motility of Flexibacter FS-1. J Bacteriol 133:1517–1519
Cottrell IW, Shim JL, Best GH (1978) Sulfonic acid and sulfomethyl-containing graft co-polymers of xanthan gum. In: Schweiger RG (ed) Carbohydrate sulfates. American Chemical Society, Washington, DC, pp 193–202
Daniels SL (1980) Mechanisms involved in sorption of microorganisms to solid surfaces. In: Bitton G, Marshall KC (eds) Absorption of microorganisms to surfaces. John Wiley and Sons, New York, pp 7–58
Dayrell-Hart B, Burchard RP (1979) Association of flexing and gliding in Flexibacter. J Bacteriol 137: 1417–1420
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Duckworth M, Yaphe W (1971a) The structure of agar. I. Fractionation of a complex mixture of polysaccharides. Carbohyd Res 16:189–197
Duckworth M, Yaphe W (1971b) Preparation of agarose by fractionation from the spectrum of polysaccharides in agar. Anal Biochem 44:636–641
Dudman WF (1978) Structural studies of the extracellular polysaccharides of Rhizobium japonicum strains 71A, CC 708 and CB 1795. Carbohyd Res 66:9–23
Foster RC (1981) Polysaccharides in soil fabrics. Science 214:665–667
Glaser J, Pate JL (1973) Isolation and characterization of gliding motility mutants of Cytophaga columnaris. Arch Mikrobiol 92:295–309
Henrichsen J (1972) Bacterial surface translocation: a survey and a classification. Bacteriol Rev 36:478–503
Humphrey BA, Dickson MR, Marshall KC (1979) Physiochemical and in situ observations on the adhesion of gliding bacteria to surfaces. Arch Microbiol 120:231–238
Jeanes A, Pittsley JE, Watson PR (1964) Exocellular polysaccharide produced from glucose by Cryptococcus laurentii var flavescens NRRL Y-1401: chemical and physical characterization. J Appl Polymer Sci 8:2775–2787
Lapidus IR, Berg HC (1982) Gliding motility of Cytophaga sp U67. J Bacteriol 151:384–398
Lewin RA (1974) Flexibacter polymorphus, a new marine species. J Gen Microbiol 82:393–403
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
McNeeley WH, Kang KS (1973) Xanthan and some other biosynthetic gums. In: Whistler RL, BeMiller JN (eds) Industrial gums. Academic Press, New York, pp 473–499
Marshall KC, Cruickshank RH (1973) Cell surface hydrophobicity and the orientation of certain bacteria at interfaces. Arch Mikrobiol 91:29–40
Novotny M, Bartle KD (1974) Surface chemistry of glass open tubular (capillary) columns in gas-liquid chromatography. Chromatographia 7:122–127
Rees DA (1972) Shapely polysaccharides. Biochem J 126:257–273
Reichenbach R (1981) Taxonomy of the gliding bacteria. Ann Rev Microbiol 35:339–364
Ridgway HF (1977) Ultrastructural characterization of goblet-shaped particles from the cell wall of Flexibacter polymorphus. Can J Microbiol 23:1201–1213
Rol F (1973) Locust bean gum. In: Whistler RL, BeMiller JN (eds) Industrial gums. Academic Press, New York, pp 323–338
Seymour FR, Slodki ME, Plattner RD, Stodola RM (1976) Methylation and acetolysis of extracellular D-mannans from yeast. Carbohyd Res 48:225–237
Simon GD, White D (1971) Growth and morphological characteristics of a species of Flexibacter. Arch Mikrobiol 78:1–16
Slodki ME, Safranski MJ, Hensley DE, Babcock GE (1970) Variation in composition of yeast phosphohexosans. Appl Microbiol 19:1019–1020
Slodki ME, Wickerham LJ, Cadmus MC (1961) Phylogeny of phosphomannan-producing yeasts. II. Phosphomannan properties and taxonomic relationships. J Bacteriol 82:269–274
Sloneker JH, Orentas DG, Knutson CA, Watson PR, Jeanes A (1968) Structure of extracellular bacterial polysaccharide from Arthrobacter viscosus NRRL B-1973. Can J Chem 46:3353–3361
Towle GA, Christensen O (1973) Pectin. In: Whistler RL, BeMiller JN (eds) Industrial gums. Academic Press, New York, pp 429–468
Whistler RL, Smart CL (1953) Polysaccharide chemistry. Academic Press, New York
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Arlauskas, J., Burchard, R.P. Substratum requirements for bacterial gliding motility. Arch. Microbiol. 133, 137–141 (1982). https://doi.org/10.1007/BF00413527
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DOI: https://doi.org/10.1007/BF00413527