Skip to main content
SpringerLink
Log in

Studies on the macroconidia of Microsporum canis

Characteristics of in vitro amino acid incorporating system

  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

The characteristics of an in vitro polyuridylic acid dependent amino acid incorporating system prepared from germinating macroconidia of Microsporum canis are described. The incorporation of 14C-phenylalanine into polyphenylalanine is dependent on S-30 extract, adenosine triphosphate, magnesium ions and polyuridylic acid. Incorporation is slightly enhanced by yeast transfer ribonucleic acid and pyruvate kinase. The system is highly sensitive to ribonuclease, puromycin and miconazole (an antifungal agent), moderately sensitive to sodium fluoride and much less sensitive to phenethylalcohol, cycloheximide, chloramphenicol and deoxyribonuclease. Cell-free extract from ungerminated conidia has less capacity to synthesize the protein and during germination a marked increase in the protein synthetic activity is observed. The results from experiments wherein ribosomes and S-100 fraction from germinated and ungerminated spores are interchanged, revealed that the defect in the extract from the ungerminated spore is in the ribosomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Poly(U):

polyuridylic acid

tRNA:

transfer ribonucleic acid

ATP:

adenosine triphosphate

GTP:

guanosine triphosphate

BSA:

bovine serum albumin

RNase:

ribonuclease

DNase:

deoxyribonuclease

POPOP:

1,4-bis-2(5-phenyl oxazolyl)benzene

PPO:

2,5-diphenyl oxazole

TCA:

trichloracetic acid

References

  • Brambl, R. M., Van Etten, J. L.: Protein synthesis during fungal spore germination. V. Evidence that the ungerminated conidiospores of Botryodiplodia theobromae contain messenger ribonucleic acid. Arch. Biochem. Biophys. 137, 442–452 (1970)

    Google Scholar 

  • Bretthauer, R. K., Marcus, L., Chaloupka, J., Halvorson, H. O., Bock, R. M.: Amino acid incorporation into protein by cell-free extracts of yeast. Biochemistry 2, 1079–1084 (1963)

    Google Scholar 

  • Cochrane, J. C., Rado, T. A., Cochrane, V. W.: Synthesis of macromolecules and polyribosome formation in early stages of spore germination in Fusarium solani. J. Gen. Microbiol. 65, 45–55 (1971)

    Google Scholar 

  • Garvey, M. C., Tewari, R. P.: A cell-free amino acid incorporating system from yeast phase cells of Histoplasma capsulatum. Sabouraudia 10, 113–121 (1972)

    Google Scholar 

  • Hahn, F. E.: Chloramphenicol. In: Antibiotics, mechanism of action. Vol. I (D. Gottlieb, P. D. Shaw, eds.), pp. 308–330. Berlin-Heidelberg-New York: Springer 1967

    Google Scholar 

  • Hollomon, D. W.: Protein synthesis during germination of Peronospora tabacina (Adam) conidia. Arch. Biochem. Biophys. 145, 643–649 (1971)

    Google Scholar 

  • Horikoshi, K., Ikeda, Y.: Studies on the conidia of Aspergillus oryzae. VIII. Development of protein synthesizing activity during germination. Biochim. Biophys. Acta 166, 505–511 (1968)

    Google Scholar 

  • Horikoshi, K., Ohtaka, Y., Ikeda, Y.: Ribosomes in dormant and germinating conidia of Aspergillus oryzae. Agric. Biol. Chem. 29, 724–727 (1965)

    Google Scholar 

  • Jaworski, A. J.: Synthesis of poly(A)-containing RNA during zoospore differentiation in Blastocladiella emersonii. Arch. Biochem. Biophys. 173, 201–209 (1976)

    Google Scholar 

  • Jimenez, A., Littlewood, B., Davies, J.: Inhibition of protein synthesis in yeast. In: Molecular mechanism of antibiotic action on protein synthesis and membranes (E. Munoz, Garcia-Ferrandez, D. Vasquez, eds.), pp. 292–306. Amsterdam: Elsevier 1972

    Google Scholar 

  • Knight, R. H., Van Etten, J. L.: Characteristics of ribonucleic acids isolated from Botryodiplodia theobromae pycnidiospores. Arch. Microbiol. 109, 45–50 (1976)

    Google Scholar 

  • Kobayashi, Y., Halvorson, H. O.: Nature of defect in the cell-free protein synthesis in extracts of bacterial spores. Bact. Proc. 1967, 22 (1967)

    Google Scholar 

  • Lamborg, M., Zamecnik, P. C.: Amino acid incorpnration into protein by extracts of Escherichia coli. Biochim. Biophys. Acta 42, 206–211 (1960)

    Google Scholar 

  • Lampen, J. O.: Amphotericin B and other polyenic antifungal antibiotics Am. J. Clin. Pathol. 52, 138–146 (1969)

    Google Scholar 

  • Lee, P. C., De Busk A. G.: Amino acid incorporation by cell-free extracts of Neurospora crassa. Biochim. Biophys. Acta 157, 427–429 (1968)

    Google Scholar 

  • Lewis, J., Raghu, K., Keswani, C. L., Weber, D. J.: Characterization of ribosomes from the phycomycetes Rhizopus arrhizus and Pythium debaryanum. Biochim. Biophys. Acta 199, 194–202 (1970)

    Google Scholar 

  • Lowry, E. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)

    Google Scholar 

  • Lucas, J. M., Schuurs, A. H. W. M., Simpson, M. V.: A cell-free amino acid-incorporating system from Saccharomyces cerevisiae. Variation in ribosomal activity and in RNA synthesis during logarithmic growth. Biochemistry 3, 959–967 (1964)

    Google Scholar 

  • Mirkes, P. G.: Polysomes, ribonucleic acid, and protein synthesis during germination of Neurospora crassa conidia. J. Bacteriol. 117, 196–202 (1974)

    Google Scholar 

  • Obrig, T. G., Cerna, J., Gottlieb, D.: Characteristics of in vitro protein synthesis systems from Rhizoctonia solani and Sclerotium bataticola. Phytopathology 59, 187–192 (1969)

    Google Scholar 

  • Pongratz, M., Klingmüller, W.: Role of ribosomes in cycloheximide resistance of Neurospora mutants. Molec. Gen. Genet. 124, 359–363 (1973)

    Google Scholar 

  • Rao, S., Grollman, A.: Cycloheximide resistance in yeast: a property of 60 S ribosomal subunit. Biochem. Biophys. Res. Commun. 29, 696–704 (1967)

    Google Scholar 

  • Schimmelpfennig, L., Grierson, D., Zetsche, K.: Preformed poly(A)-containing RNA in zoospores of Blastocladiella emersonii. Biochim. Biophys. Acta 435, 340–348 (1976)

    Google Scholar 

  • Schmoyer, I. R., Lovett, J. S.: Regulation of protein synthesis in zoospores of Blastocladiella. J. Bacteriol. 100, 854–864 (1969)

    Google Scholar 

  • Scragg, A. H., Morimoto, H., Villa, V., Nekhoronocheff, J., Halvorson, H. O.: Cell-free protein synthesizing system from yeast mitochondria. Science 171, 908–910 (1971)

    Google Scholar 

  • Siegel, M. R., Sisler, H. D.: Site of action of cycloheximide in cells of Saccharomyces pastorianus. II. The nature of inhibition of protein synthesis in a cell-free system. Biochim. Biophys. Acta 87, 83–89 (1964)

    Google Scholar 

  • So, A. G., Davie, E. W.: The incorporation of amino acids into protein in a cell-free system from yeast. Biochemistry 2, 132–136 (1963)

    Google Scholar 

  • Sreedharaswamy, K. H., Ramananda Rao G.: Studies on the mechanism of action of miconazole. II. Interaction of miconazole with mammalian erythrocytes. Biochem. Pharmacol. 25, 1145–1150 (1975)

    Google Scholar 

  • Sreedharaswamy, K. H., Sirsi, M., Ramananda Rao, G.: Studies on mechanism of action miconazole: Effect of miconazole on respiration and cell permeability of Candida albicans. Antimicrob. Agents Chemother. 5, 420–425 (1974)

    Google Scholar 

  • Staples, R. C., App, A. A., McCarthy, W. J., Gerosa, M. M.: Some properties of ribosomes from uredospores of the bean rust fungus. Contrib. Boyce Thompson Inst. 23, 159–164 (1966)

    Google Scholar 

  • Van Etten, J. L.: Protein synthesis during fungal spore germination. A. Characteristics of an in vitro phenylalanine incorporating system prepared from germinated spores of Botryodiplodia theobromae. Arch. Biochem. Biophys. 125, 13–21 (1968)

    Google Scholar 

  • Vijaya Manohar, Sirsi, M., Ramananda Rao, G.: Studies on superficial mycoses. Arogya-J. Health Sci. III, 31–40 (1977)

    Google Scholar 

  • Yamaguchi, H., Iwata, K.: In vitro and in vivo protein synthesis in Candida albicans. I. Isolation and protein synthesis of polysomes. Sabouraudia 8, 177–189 (1970)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560012, Bangalore, India

    B. M. Jayaram & G. Ramananda Rao

Authors
  1. B. M. Jayaram
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Ramananda Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jayaram, B.M., Rao, G.R. Studies on the macroconidia of Microsporum canis . Arch. Microbiol. 120, 67–72 (1979). https://doi.org/10.1007/BF00413276

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00413276

Key words

Search

Navigation