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Isolation and characterization of mutant Pseudomonas aeruginosa strains unable to assimilate nitrate

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Abstract

Single-site mutants of Pseudomonas aeruginosa that lack the ability aerobically to assimilate nitrate and nitrite as sole sources of nitrogen have been isolated. Twentyone of these have been subdivided into four groups by transductional analysis. Mutants in only one group, designated nis, lost assimilatory nitrite reductase activity. Mutants in the other three transductional groups, designated ntmA, ntmB, ntmC, display a pleiotropic phenotype: utilization of a number of nitrogen-containing compounds including nitrite as sole nitrogen sources is impaired. Assimilatory nitrite reductase was shown to be the major route by which hydroxylamine is reduced in aerobically-grown cells.

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References

  • Adams MH (1959) Bacteriophages. Wiley-Interscience, New York, p 592

    Google Scholar 

  • Berg R, Becker E (1940) Ein neuer Nachweis von Hydroxylamin durch Bildung von Chinolinchinon-(5.8)-[8-oxy-chinolyl-5-imid]-(5), genannt “Indo-oxin”. Chem Ber 73:172–173

    Google Scholar 

  • Brown CM, Macdonald-Brown DS, Stanley SO (1973) The mechanism of nitrogen assimilation in pseudomonads. An van Leeuwenhoek J Microbiol Serol 39:89–98

    Google Scholar 

  • Bussey LB, Ingraham JL (1982) A regulatory gene (use) affecting the expression of pyrA and certain other pyrimidine genes. J Bacteriol 151:144–152

    Google Scholar 

  • Carlson CA, Ferguson LP, Ingraham JL (1982) Properties of dissimilatory nitrate reductase purified from the denitrifier Pseudomonas aeruginosa. J Bacteriol 151:162–171

    Google Scholar 

  • Cove JD (1979) Genetic studies of nitrate assimilation in Aspergillus nidulans. Biol Rev 54:291–327

    Google Scholar 

  • Delwiche CC, Bryan BA (1976) Denitrification. Ann Rev Microbiol 30:241–262

    Google Scholar 

  • Focht DD, Verstraete W (1977) Biochemical ecology of nitrification and denitrification. Adv Microbial Ecol 1:135–214

    Google Scholar 

  • Frear DS, Burrell RC (1955) Spectrophotometric method for determining hydroxylamine reductase activity in higher plants. Anal Chem 27:1664–1665

    Google Scholar 

  • Garrett RH, Amy NK (1978) Nitrate assimilation in fungi. Adv Microbial Physiol 18:1–65

    Google Scholar 

  • Holloway BW, Egan JB, Monk M (1960) Lysogeny in Pseudomonas aeruginosa. Aust J Exptal Biol 38:321–330

    Google Scholar 

  • Jacoby GA (1974) Properties of R plasmids determining gentamicin resistance by acetylation in Pseudomonas aeruginosa. Antimicr Agents Chemother 6:239–252

    Google Scholar 

  • Janssen DB, Camp HJM op den, Leenen PJM, Drift C van der (1980) The enzymes of the ammonia assimilation in Pseudomonas aeruginosa. Arch Microbiol 124:197–203

    Google Scholar 

  • Janssen DB, Habets WJA, Marugg JT, Drift C van der (1982) Nitrogen control in Pseudomonas aeruginosa: mutants affected in the synthesis of glutamine synthetase, urease, and NADP-dependent glutamate dehydrogenase. J Bacteriol 151:22–28

    Google Scholar 

  • Knowles R (1982) Denitrification. Microbiol Rev 46:43–70

    Google Scholar 

  • Lafferty MA, Garrett RH (1974) Purification and properties of the Neurospora crassa assimilatory nitrite reductase. J Biol Chem 249:7555–7567

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Medina A, Nicholas DJD (1957) Hyponitrite reductase in Neurospora. Nature (London) 179:533–534

    Google Scholar 

  • Merrick MJ (1982) A new model for nitrogen control. Nature 297:362–363

    Google Scholar 

  • Meynell GG, Meynell E (1970) Theory and practice in experimental bacteriology, 2nd edn. Cambridge University Press, Cambridge, p 347

    Google Scholar 

  • Nyberg K, Clarke PH (1978) Glutamine synthetase activities of cultures of Pseudomonas aeruginosa grown in minimal media with histidine, nitrate or ammonium sulphate as the nitrogen source. J Gen Microbiol 107:193–197

    Google Scholar 

  • Payne WJ (1973) Reduction of nitrogenous oxides by microorganisms. Bacteriol Rev 37:409–452

    Google Scholar 

  • Pemberton JM, Clark AJ (1973) Detection and characterization of plasmids in Pseudomonas aeruginosa strain PAO. J Bacteriol 114:424–433

    Google Scholar 

  • Sias SR, Ingraham JL (1979) Isolation and analysis of mutants of Pseudomonas aeruginosa unable to assimilate nitrate. Arch Microbiol 122:263–270

    Google Scholar 

  • Sias SR, Stouthamer AH, Ingraham JL (1980) The assimilatory and dissimilatory nitrate reductases of Pseudomonas aeruginosa are encoded by different genes. J Gen Microbiol 118:229–234

    Google Scholar 

  • Siegel LM, Leinweber F-J, Monty KJ (1965) Characterization of the sulfite and hydroxylamine reductases of Neurospora crassa. J Biol Chem 240:2705–2711

    Google Scholar 

  • Spencer D, Takahashi H, Nason A (1957) Relationship of nitrite and hydroxylamine reductases to nitrate assimilation and nitrogen fixation in Azotobacter agile. J Bacteriol 73:553–562

    Google Scholar 

  • Vega JM, Guerrero MG, Leadbetter E, Losada M (1973) Reduced nicotinamide adenine dinucleotide-nitrite reductase from Azotobacter chroococcum. Biochem J 133:701–708

    Google Scholar 

  • Vega JM, Kamin H (1977) Spinach nitrite reductase. Purification and properties of a siroheme-containing iron-sulfur enzyme. J Biol Chem 252:896–909

    Google Scholar 

  • Whiting PH, Midgley M, Dawes EA (1976) The role of glucose limitation in the regulation of the transport of glucose, gluconate and 2-oxogluconate, and of glucose metabolism in Pseudomonas aeruginosa. J Gen Microbiol 92:304–310

    Google Scholar 

  • Zumft WG (1972) Ferredoxin-nitrite oxidoreductase from Chlorella. Purification and properties. Biochim Biophys Acta 276:363–375

    Google Scholar 

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In memoriam of Professor R. Y. Stanier

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Jeter, R.M., Ingraham, J.L. Isolation and characterization of mutant Pseudomonas aeruginosa strains unable to assimilate nitrate. Arch. Microbiol. 138, 124–130 (1984). https://doi.org/10.1007/BF00413012

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  • DOI: https://doi.org/10.1007/BF00413012

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