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Membranpotentiale bei experimentellem Kaliummangel

Messungen am Rattenzwerchfell in vitro

Membrane potentials in experimental potassium deficiency

Measurements in rat diaphragm in vitro

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Summary

In 127 skeletal muscle cells of 8 rats with alimentary potassium depletion we found a membrane potential of −92.8 (s=±6.8) mV. This value is 12.4 mV higher than the membrane potential of −80.4 (s=±6.5) mV in 150 cells of 10 control animals (p<0.001). The measurements were made in diaphragm muscles incubated in a modified Krebs-solution. The found hyperpolarisation can not be explained by change of the intra-/extracellular potassium concentrationgradient, but has to be regarded as a consequence of ionic conductivity changes of the cell membrane, possibly as a consequence of a stimulated active sodium transport (“electrogenic pump”).

Zusammenfassung

Bei insgesamt 127 Zellen von 8 Ratten mit einem alimentären Kaliummangel fanden wir mit −92,8 (s=±6,8) mV ein absolut um 12,4 mV höheres Membranpotential (p<0,001) als bei insgesamt 150 Zellen von 10 Kontrolltieren mit einem Wert von −80,4 (s=±6,5) mV. Die Untersuchungen wurden in vitro bei gleicher extracellulärer Kaliumkonzentration durchgeführt. Die gemessene Hyperpolarisation kann nicht durch eine Änderung des intraextracellulären Kaliumkonzentrationsgradienten erklärt werden. Sie muß als Folge von Änderungen der Ionenleitfähigkeit der Zellmembran angesehen werden, möglicherweise im Sinne einer Stimulierung des aktiven Natriumtransports („elektrogene Pumpe“).

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Authors and Affiliations

  1. I. Medizinische Klinik der Universität München, Germany

    H.-D. Bolte & B. Lüderitz

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  1. H.-D. Bolte
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  2. B. Lüderitz
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Mit Unterstützung der Deutschen Forschungsgemeinschaft.

Technische Assistenz: S. Winterling.

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Bolte, HD., Lüderitz, B. Membranpotentiale bei experimentellem Kaliummangel. Pflügers Arch. 301, 43–49 (1968). https://doi.org/10.1007/BF00412417

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  • DOI: https://doi.org/10.1007/BF00412417

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