Summary
The tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate (TPA) increases by severalfold the synthesis of Mason-Pfizer monkey virus (MPMV), a type D retrovirus, when the virus is growing in human embryo kidney (HEK) cells. The effect is transient and paralleled by a striking morphological alteration of the cells. The optimal TPA concentration for stimulation is 5 ng·ml-1. Contrary to infected HEK cells, TPA induces at similar concentrations neither stimulation of MPMV synthesis nor altered morphology in persistently MPMV-infected cells of the continuous human tumor cell line A 204.
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Abbreviations
- MPMV:
-
Mason-Pfizer monkey virus
- TPA:
-
12-0-tetradecanoyl-phorbol-13-acetate
- HEK:
-
human embryo kidney
- RT:
-
reverse transcriptase
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Dedicated to Prof. A. Graffi, MD, on the occasion of his 70th birthday
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Wunderlich, V., Baumbach, L. & Sydow, G. Tumor promoter-stimulated synthesis of Mason-Pfizer monkey virus. J Cancer Res Clin Oncol 102, 271–275 (1982). https://doi.org/10.1007/BF00411347
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DOI: https://doi.org/10.1007/BF00411347