Summary
Fibroblast cultures from 29 lung cancer patients including eight familial cases and from 25 healthy controls were investigated. We studied the benzpyrene-induced sister chromatid exchange (SCE) following incubation in the presence of 150 nM benzo(a)pyrene and found spontaneously increased SCE values in the patients' cells but a reduced response to benzprene as compared to the control cultures. The benzypyrene-induced SCE was positively correlated with both benzpyrene metabolism and DNA adduts in normal control cells but not in the patients' cells.The ratio of benzpyrene DNA adducts and benzpyrene-induced rate of SCE calculated for the individual cell strains exhibited a bimodal distribution with patients' cells, but not with control cells. We speculate that the described differences between patients' and control fibroblasts might reflect a genetic predisposition to the development of lung cancer in about one half of the patients tested.
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Part of a thesis of W.H. for a medical doctorate
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Rüdiger, H.W., Harder, W., Maack, P. et al. Decreased rate of benzo(a)pyrene-induced sister chromatid exchange in fibroblast cultures from patients with lung cancer. J Cancer Res Clin Oncol 102, 169–175 (1981). https://doi.org/10.1007/BF00410668
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DOI: https://doi.org/10.1007/BF00410668