Abstract
Isolated collagen fibrils treated with type-specific antibodies can be stained using a peroxidase-antibody complex. The staining pattern of the fibrils with this peroxidase-antibody complex is demonstrated and compared with the normal negative staining pattern of the fibrils. The possible binding sites of the anticollagen antibodies on the fibrils is also discussed.
Similar content being viewed by others
References
Barrach, H.J., Angermann, K.: Immunofluorescence as a tool in teratological research (localisation of collagen types in fetal tissues by immunofluorescence). In: Methods in Prenatal Toxicology, edited by Neubert, D., Merker, H.-J. and Kwasigroch, T.E., pp. 332–347, Stuttgart: Thieme 1977
Chapman, J.A.: The staining pattern of collagen fibrils. I. Connective Tissue Res. 2, 137–150 (1974)
Harnisch, J.-P., Buchen, R., Sinha, P.K., Barrach, H.J.: Ultrastructural identification of type I and II collagen in the cornea of the mouse by means of enzyme labeled antibodies. A. von Graefes Arch. klin. Exp. Ophthal. 208, 9–13 (1978)
Orekhovitch, V.N., Shpikiter, V.O.: Science 127, 1371–1385 (1958)
Timpl, R., Wick, G., Gay, S.: Antibodies to distinct types of collagens and procollagens and their application in immunohistology. Z. Immunol. Meth. 18, 165–182 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Harnisch, J.P., Barrach, H.J. & Sinha, P.K. Immunoelectronmicroscopical investigations on isolated collagen fibrils. Albrecht von Graefes Arch. Klin. Ophthalmol. 212, 143–148 (1980). https://doi.org/10.1007/BF00410510
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00410510