Skip to main content
Log in

Immunoelectronmicroscopical investigations on isolated collagen fibrils

  • Published:
Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Aims and scope Submit manuscript

Abstract

Isolated collagen fibrils treated with type-specific antibodies can be stained using a peroxidase-antibody complex. The staining pattern of the fibrils with this peroxidase-antibody complex is demonstrated and compared with the normal negative staining pattern of the fibrils. The possible binding sites of the anticollagen antibodies on the fibrils is also discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barrach, H.J., Angermann, K.: Immunofluorescence as a tool in teratological research (localisation of collagen types in fetal tissues by immunofluorescence). In: Methods in Prenatal Toxicology, edited by Neubert, D., Merker, H.-J. and Kwasigroch, T.E., pp. 332–347, Stuttgart: Thieme 1977

    Google Scholar 

  • Chapman, J.A.: The staining pattern of collagen fibrils. I. Connective Tissue Res. 2, 137–150 (1974)

    Google Scholar 

  • Harnisch, J.-P., Buchen, R., Sinha, P.K., Barrach, H.J.: Ultrastructural identification of type I and II collagen in the cornea of the mouse by means of enzyme labeled antibodies. A. von Graefes Arch. klin. Exp. Ophthal. 208, 9–13 (1978)

    Google Scholar 

  • Orekhovitch, V.N., Shpikiter, V.O.: Science 127, 1371–1385 (1958)

    Google Scholar 

  • Timpl, R., Wick, G., Gay, S.: Antibodies to distinct types of collagens and procollagens and their application in immunohistology. Z. Immunol. Meth. 18, 165–182 (1977)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Harnisch, J.P., Barrach, H.J. & Sinha, P.K. Immunoelectronmicroscopical investigations on isolated collagen fibrils. Albrecht von Graefes Arch. Klin. Ophthalmol. 212, 143–148 (1980). https://doi.org/10.1007/BF00410510

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00410510

Keywords

Navigation