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Formation of pyrophosphate during ATP: Glutamine synthetase-adenylyltransferase-reaction in E. coli

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Summary

The ATP:glutamine synthetase adenylyltransferase reaction was studied with γ-32P-labelled ATP.ATP, phosphate, and pyrophosphate were separated by electrophoresis and the radioactivity of these substances was determined quantitatively. For each mole of AMP covalently attached to glutamine synthetase, one mole of pyrophosphate is formed. The equation for the reaction is therefore:

$$\begin{gathered} 12 ATP + glutamine synthetase \xrightarrow{{Mg + + , glutamine}} \hfill \\ glutamine synthetase( - AMP)_{12} + 12 pyrophosphate. \hfill \\ \end{gathered}$$

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Authors and Affiliations

  1. Biochemisches Institut der Universität Freiburg i. Br., Freiburg i. Br, Deutschland

    C. Peter Heinrich, Frank A. Battig, Michael Mantel & Helmut Holzer

  2. Sandoz Forschungsinstitut Wien, Austria

    C. Peter Heinrich, Frank A. Battig, Michael Mantel & Helmut Holzer

Authors
  1. C. Peter Heinrich
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  2. Frank A. Battig
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  3. Michael Mantel
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  4. Helmut Holzer
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Heinrich, C.P., Battig, F.A., Mantel, M. et al. Formation of pyrophosphate during ATP: Glutamine synthetase-adenylyltransferase-reaction in E. coli . Archiv. Mikrobiol. 73, 104–110 (1970). https://doi.org/10.1007/BF00410313

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  • DOI: https://doi.org/10.1007/BF00410313

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