Summary
In Nocardia opaca strain 1b the key enzymes of the Entner-Doudoroff pathway, 6-phosphogluconate dehydrase and 2-keto-3-deoxy-6-phosphogluconate aldolase, are inducible, while glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase are constitutively formed. In autotrophically grown cells the fructose diphosphate aldolase has six-fold specific activity compared to fructose- or gluconate-grown cells. Molecular hydrogen represses the adaptation to fructose, glucose, or gluconate and inhibits the utilization of glucose or fructose.
Glucose-6-phosphate dehydrogenase has been purified 22fold. It utilizes either NADP of NAD as coenzyme; it requires magnesium ions for maximal activity. The dehydrogenation reaction is competitively inhibited by rather high concentrations (2–6 mM) of ATP, and in a similar fashion by phosphoenolpyruvate. Homotropic or heterotropic interactions between substrate molecules were not detected. The effect of ATP is relieved by equimolar concentrations of magnesium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aggag, M., Schlegel, H. G.: Studies on a Gram-positive hydrogen bacterium, Nocardia opaca strain 1b. Arch. Mikrobiol. 88, 299–318 (1973).
Andreesen, J. R., Gottschalk, G.: The occurrence of a modified Entner-Doudoroff pathway in Clostridium aceticum. Arch. Mikrobiol. 69, 160–170 (1969).
Beisenherz, G., Bolze, H. J., Bücher, Th., Czok, R., Garbade, K. H., Meyer-Arendt, E., Pfleiderer, G.: Diphosphofructose-Aldolase, Phosphoglyceraldehyd-Dehydrogenase, Milchsäure-Dehydrogenase, Glycerophosphat-Dehydrogenase und Pyruvat-Kinase aus Kaninchenmuskulatur in einem Arbeitsgang. Z. Naturforsch. 8b, 555–577 (1953).
Bender, R.: Anreicherung und Charakterisierung der d-Gluconat-Dehydratase von Clostridium pasteurianum. Diss., Göttingen 1972.
Blackkolb F., Schlegel, H. G.: Katabolische Repression und Enzymhemmung durch molekularen Wasserstoff bei Hydrogenomonas. Arch. Mikrobiol. 62, 129–143 (1968a).
Blackkolb, F., Schlegel, H. G.: Regulation der Glucose-6-phosphat-Dehydrogenase aus Hydrogenomonas H 16 durch ATP und NADH2. Arch. Mikrobiol. 63, 177–196 (1968b).
Bowien, B.: Hexoseabbau und Pentosebiosynthese in Hydrogenomonas. Diss., Göttingen 1970.
Bowien, B., Schlegel, H. G.: Der Biosyntheseweg der RNS-Ribose in Hydrogenomonas Stamm H 16 und Pseudomonas facilis. Arch. Mikrobiol. 85, 95–112 (1972).
Eisenberg, R. C., Dobrogosz, W. J.: Gluconate metabolism in Escherichia coli. J. Bact. 93, 941–949 (1967).
Gottschalk, G.: Die Verwertung organischer Substrate durch Hydrogenomonas in Gegenwart von molekularem Wasserstoff. Biochem. Z. 341, 260–270 (1965).
Gottschalk, G., Eberhardt, U., Schlegel, H. G.: Verwertung von Fructose durch Hydrogenomonas H 16. Arch. Mikrobiol. 48, 95–108 (1964).
Lessie, T., Neidhardt, F. C.: ATP-linked control of Pseudomonas aeruginosa glucose-6-phosphate dehydrogenase. J. Bact. 93, 1337–1345 (1967).
Lessie, T. G., Van der Wyk, J. C.: Multiple forms of Pseudomonas multivorans glucose-6-phosphate and 6-phosphogluconate dehydrogenases: Differences in size, pyridine nucleotide specificity, and susceptibility to inhibition by adenosine 5′-triphosphate. J. Bact. 110, 1107–1117 (1972).
Ling, K. H., Byme, W. L., Laroly, H.: Phosphohexokinase. In: Methods in enzymology, S. P. Colowick and N. O. Kaplan, eds., Vol. I, pp. 306–310. New York: Academic Press 1955.
Ohmann, E., Rindt, K. R., Borris, R.: Glucose-6-phosphat-Dehydrogenase in autotrophen Mikroorganismen. I. Regulation der Synthese in Euglena gracilis und Rhodopseudomonas spheroides in Abhängigkeit von den Kulturbedingungen. Z. allg. Mikrobiol. 9, 557–564 (1969).
Racker, E., Schroeder, E. A. R.: The reductive pentosephosphate cycle. II. Specific c1-phosphatases for fructose-1.6-diphosphate and seduheptulose-1.7-diphosphate. Arch. Biochem. 74, 326–344 (1958).
Schlegel, H. G., Blackkolb, F.: Regulation des Hexoseabbaus über den Entner-Doudoroff-Weg durch Enzymhemmung. Nachr. Akad. Wiss. Göttingen, II. Math. Physik. Kl. 1967, 51–60.
Schlegel, H. G., Eberhardt, U.: Regulatory phenomena in the metabolism of Knallgasbacteria. Advanc. Microbiol. Physiol. 7, 205–242 (1972).
Schlegel, H. G., Kaltwasser, H., Gottschalk, G.: Ein Submersverfahren zur Kultur wasserstoffoxidierender Bakterien: Wachstumsphysiologische Untersuchungen. Arch. Mikrobiol. 39, 209–222 (1961).
Schlegel, H. G., Trüper, H. G.: Repression of enzyme formation in Hydrogenomonas strain H 16 G+ by molecular H2 and fructose. Antonie v. Leeuwenhoek 32, 277–292 (1966).
Siebert, D.: Über propanverwertende, wasserstoffoxidierende Bakterien und die Charakterisierung eines Förderungsfaktors. Diss., Göttingen 1969.
Szymona, M., Doudoroff, M.: Carbohydrate metabolism in Rhodopseudomonas spheroides. J. gen. Microbiol. 22, 167–183 (1960).
Warburg, O., Christian, W.: Isolierung und Kristallisation des Gärungsfermentes Enolase. Biochem. Z. 310, 384–421 (1941).
Wilde, E.: Untersuchungen über Wachstum und Speicherstoff-Synthese von Hydrogenomonas. Arch. Mikrobiol. 43, 109–137 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Probst, I., Schlegel, H.G. Studies on a gram-positive hydrogen bacterium, Nocardia opaca strain 1b . Archiv. Mikrobiol. 88, 319–330 (1973). https://doi.org/10.1007/BF00409943
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00409943