Abstract
The effects of solar UV-B radiation on the green flagellate, Euglena gracilis, are measured under controlled conditions. Both photoorientation and motility are drastically impaired even after short exposure times of a few hours to sunlight not filtered by an ozone cuvette. Phototactic orientation starts to deteriorate after about 90 min and is completely lost after about 5 h. The percentage of motile cells in a population decreases likewise after an exposure of about 2 h and the velocity distributions shows a reduced speed of movement after an initial photokinetic increase. The damage is irreversible: in populations exposed for >2 h no living cell was found 24 h later. The UV-B sensitivity seems to be independent of the culture age at least over three weeks: While the percentage of motile cells changes with a peak at about 8 d, the relative UV-B induced inhibition is constant and depends only on the UV dose. DNA seems not to be the primary UV-B target since UV-B inhibition could not be repaired during subsequent dark or moderate light conditions even after low doses.
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References
Bancroft FW (1913) Heliotropism, differential sensibility and galvanotropism in Euglena. J Exp Zool 15:383–420
Batschelet E (1965) Statistical methods for the analysis of problems in animal orientation and certain biological rhythms. In: Galles SR, Schmidt-Koenig K, Jacobs GJ, Belleville RF (eds) Animal orientation and navigation. NASA, Washington, pp 61–91
Batschelet E (1981) Circilar statistics in biology. Academic Press, London
Benedetti PA, Checcucci A (1975) Paraflagellar body (PFB) pigments studied by fluorescence microscopy in Euglena gracilis. Plant Sci Lett 4:47–51
Checcucci A, Colombetti G, Ferrara R, Lenci F (1976) Action spectra for photoaccumulation of green and colorless Euglena: evidence for identification of receptor pigments. Photochem Photobiol 23:51–54
Checcucci A, Favati L, Grassi S, Piaggesi T (1975) The measurement of phototactic activity in Euglena gracilis Klebs. Monit Zool Ital 9:83–98
Colombetti G, Lenci F, Diehn B (1982) Responses to photic, chemical and mechanical stimuli. In: Buetow DE (ed) The biology of Euglena, vol III. Academic Press, New York, pp 169–195
Creutz C, Diehn B (1976) Motor responses to polarized light and gravity sensing in Euglena gracilis. J Protozool 23:552–556
Diehn B (1969) Action spectra of the phototactic responses in Euglena. Biochim Biophys Acta 177:136–143
Doughty MJ, Diehn B (1982) Photosensory transduction in the flagellated alga, Euglena gracilis. III. Induction of Ca2+-dependent responses by monovalent cation ionophores. Biochim Biophys Acta 682:32–43
Doughty MJ, Diehn B (1983) Photosensory transduction in the flagellated alga, Euglena gracilis. IV. Long term effects of ions and pH on the expression of step-down photobehavior. Arch Microbiol 134:204–207
Doughty MJ, Diehn B (1984) Anion sensitivity of motility and step-down photophobic responses of Euglena gracilis. Arch Microbiol 138:329–332
Eker APM (1983) Photorepair processes. In: Montagnoli G, Erlanger BF (eds) Molecular models of photoresponsiveness. Plenum, New York, pp 109–132
Galbally LE (1976) Man-made carbon tetrachloride in the atmosphere. Science 111:619–624
Ghetti F, Colombetti G, Lenci F, Campani E, Polacco E, Quaglia M (1985) Fruorescence of Euglena gracilis photoreceptor pigment: an in vivo microspectrofluorimetric study. Photochem Photobiol 42:29–33
Häder D-P (1985) Effects of UV-B on motility and photobehavior in the green flagellate, Euglena gracilis. Arch Microbiol 141:159–163
Häder D-P (1986) Effects of solar and artificial UV irradiation on motility and phototaxis in the flagellate, Euglena gracilis. Photochem Photobiol 44:651–656
Häder D-P (1987) Polarotaxis, gravitaxis and vertical phototaxis in the green flagellate, Euglena gracilis. Arch Microbiol 147:179–183
Häder D-P, Griebenow K (1988a) Orientation of the green flagellate, Euglena gracilis, in a vertical column of water. FEMS Microbiol Lett (in press)
Häder D-P, Griebenow K (1988b) Versatile digital image analysis by microcomputer to count microorganisms. EDV Med Biol 18:37–42
Häder D-P, Lebert M (1985) Real time computer-controlled tracking of motile microorganisms. Photochem Photobiol 42:509–514
Häder D-P, Lipson ED (1986) Fourier analysis of angular distributions for motile microorganisms. Photochem Photobiol 44:657–663
Häder D-P, Colombetti G, Lenci F, Quaglia M (1981) Phototaxis in the flagellates, Euglena gracilis and Ochromonas danica. Arch Microbiol 130:78–82
Häder D-P, Lebert M, DiLena MR (1986a) New evidence for the mechanism of phototactic orientation of Euglena gracilis. Curr Microbiol 14:157–163
Häder D-P, Watanabe M, Furuya M (1986b) Inhibition of motility in the cyanobacterium, Phormidium uncinatum, by solar and monochromatic UV irradiation. Plant Cell Physiol 27:887–894
Häder D-P, Lebert M, DiLena MR (1987) Effects of culture age and drugs on phototaxis in the green flagellate Euglena gracilis. Plant Physiol (Life Sci Advances) 6:169–174
Hirosawa T, Miyachi S (1983) Inactivation of Hill reaction by longwavelength ultraviolet radiation (UV-A) and its photoreactivation by visible light in the cyanobacterium, Anacystis nidulans. Arch Microbiol 135:98–102
Jagger J (1983) Effects of near-UV radiation on bacteria. In: Smith KC (ed) Photochemical and photobiological reviews. Plenum, New York, pp 1–75
Jennings HS (1906) The behavior of lower organisms, 1962 edn. Univ Press Indiana, pp 134–141
Jorns MS, Sancar GB, Sancar A (1984) Studies on Escherichia coli DNA photolyase: Role of flavin in DNA repair. In: Bray RC, Engel PC, Mayhew SG (eds) Flavins and flavoproteins. Walter de Gruyter, Berlin, pp 861–874
Lenci F, Häder D-P, Colombetti G (1984) Photosensory responses in freely motile microorganisms. In: Colombetti G, Lenci F (eds) Membranes and sensory transduction. Plenum, New York, pp 199–229
Mardia KV (1972) Statistics of directional data. Academic Press, London
Mast SO (1911) Light and behavior of organisms. John Wiley & Sons, New York: Chapman & Hall, London
Molina MJ, Rowland FS (1974) Stratospheric sink of chlorofluoromethanes chlorine atoms catalyzed destruction of ozone. Nature (Lond) 249:810–812
Starr RC (1964) The culture collection of algae at Indiana University. Am J Bot 51:1013–1044
Wolken JJ, Shin E (1958) Photomotion in Euglena gracilis, I. Photokinesis. II. Phototaxis. J Protozool 5:39–46
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Häder, DP., Häder, M.A. Inhibition of motility and phototaxis in the green flagellate, Euglena gracilis, by UV-B radiation. Arch. Microbiol. 150, 20–25 (1988). https://doi.org/10.1007/BF00409712
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DOI: https://doi.org/10.1007/BF00409712