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Isolation and study of the enzymes involved in the metabolism of hydrocarbons by Candida tropicalis

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Summary

In cell free extracts prepared from protoplasts of n-tetradecane grown cells of Candida tropicalis we have found an ATP and NAD+ dependent alkane-dehydrogenase, an alcohol-dehydrogenase, an aldehyde-dehydrogenase and acyl-CoA synthetases. The study of these enzymes and their regulation allows us to propose a scheme of degradation of n-decane.

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References

  • Azoulay, E.: Métabolisme microbien des hydrocarbures à chaine droite. Thèse Faculté des Sciences. Marseille 1966.

    Google Scholar 

  • —, Chouteau, J., Daridovice, G.: Isolement et caractérisation des enzymes responsables de l'oxydation des hydrocarbures. Biochim. biophys. Acta (Amst.) 77, 554–567 (1963).

    Article  Google Scholar 

  • —, Couchoud-Beaumont, P., Senez, J. C.: Croissance de Candida lipolytica à partir des glucides et à partir hydrocarbures paraffiniques. Ann. Inst. Pasteur 107, 520–533 (1964).

    Google Scholar 

  • —, Heydeman, M. T.: Extraction and properties of alcohol dehydrogenase from Pseudomonas aeruginosa. Biochim. biophys. Acta (Amst.) 73, 1–6 (1963).

    Article  Google Scholar 

  • Baptist, J. N., Gholson, R. K., Coon, M. J.: Hydrocarbon oxidation by a bacterial enzyme system: I. Products of octane oxydation. Biochim. biophys. Acta (Amst.) 69, 40–47 (1963).

    Article  Google Scholar 

  • Chouteau, J., Azoulay, E., Senez, J. C.: Anaerobic formation of n-hept-1-ene from n-heptane by resting cells of Pseudomonas aeruginosa. Nature (Lond.) 194, 577–578 (1962).

    Google Scholar 

  • Gholson, R. K., Baptist, J. N., Coon, M. J.: Hydrocarbon oxidation by a bacterial enzyme system. II. Cofactor requirements for octanol formation from octane. Biochemistry 2, 1155–1159 (1963).

    Google Scholar 

  • Iizuka, H., Iida, M., Toyoda, S.: n-decane dehydrogenation by a cell free extract of Candida rugosa. Z. allg. Mikrobiol. 8, 145–149 (1968).

    PubMed  Google Scholar 

  • Kornberg, A., Pricer, W. E.: Enzymatic synthesis of the coenzyme A derivatives of long chain fatty acids. J. biol. Chem. 207, 329–343 (1953).

    Google Scholar 

  • Kováč, L., Bednárová, H., Greksák, M.: Oxidative phosphosphorylation in yeast. I.Isolation and properties of phosphorylating mitochondria from stationary phase cells. Biochim. biophys. Acta (Amst.) 153, 32–42 (1968).

    Google Scholar 

  • Leavitt, R. I.: Enzymatic oxidation of n-decane by a species of Achromobacter. Bact. Proc. 38, 7 (1966).

    Google Scholar 

  • Lebeault, J. M., Roche, B., Duvnjak, Z., Azoulay, E.: Protoplasts obtained from Candida tropicalis grown on alkanes. J. Bact. 100, 1218–1221 (1969).

    PubMed  Google Scholar 

  • Lipmann, P., Tuttle, L. C.: Lipase-catalysed condensation of fatty acids with hydroxylamine. Biochim. biophys. Acta (Amst.) 4, 301–309 (1950).

    Article  Google Scholar 

  • Mitchell, M. P., Hübscher, C.: Oxidation of n-hexadecane by subcellular preparations of Guinea pig small intestive. Europ. J. Biochom. 7, 90–95 (1968).

    Google Scholar 

  • Peterson, J. A., Kusunose, M., Kusunose, E., Coon, M. J.: Enzymatic ω-oxidation: II. Function of rubredoxin as the electron carrier in ω-hydroxylation. J. biol. Chem. 242, 4334–4340 (1967).

    PubMed  Google Scholar 

  • Roche, B., Azoulay, E.: Regulation des alcooldeshydrogénases chez Saccharomyces cerevisiae. Europ. J. Biochem. 8, 426–434 (1969).

    PubMed  Google Scholar 

  • Senez, J. C., Azoulay, E.: Deshydrogenation d'hydrocarbures paraffiniques par les suspensions non-proliférantes et les extraits de Pseudomonas aeruginosa. Biochim. biophys. Acta (Amst.) 47, 307–316 (1960).

    Article  Google Scholar 

  • Stewart, J. E., Kallio, R. E., Stevenson, D. P., Jones, A. C., Schissler, D. O.: Bacterial hydrocarbon oxidation. I. Oxidation of n-hexadecane by a gramnegative coccus. J. Bact. 78, 441–448 (1959).

    PubMed  Google Scholar 

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Lebeault, J.M., Roche, B., Duvnjak, Z. et al. Isolation and study of the enzymes involved in the metabolism of hydrocarbons by Candida tropicalis . Archiv. Mikrobiol. 72, 140–153 (1970). https://doi.org/10.1007/BF00409520

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