Summary
Cell-free preparations from T. neapolitanus catalyzed an ATP-dependent reduction of pyridine nucleotides by thiosulfate. The reduction of flavins by thiosulfate was also observed to be an energy-linked process. Optimal reaction occurred at pH 7.3–7.5 in the presence of 7 mM S2O3 =, 1.5 mM ATP and 0.7 mM NAD+ or NADP+. The enzyme(s) catalyzing the energy-linked reactions appear to reside in the 144000 x g supernatant fraction since washed particles failed to catalyze the ATP driven NAD+ reduction by S2O3 +; the cell-free preparations contained, however, S2O3 = oxidase and ferro-cytochrome c: O2 oxidoreductase activities. The ATP-driven reduction of flavins or that of the pyridine nucleotides was inhibited bythe inhibitors that intersect the electron transport chain in the flavin or that of the cytochrome b and c regions. In the flavin-inhibited system, quinones could substitute as electron bypass carriers for the reduction of pyridine nucleotides. Uncouplers of oxidative phosphorylation and oligomycin inhibited the energy-transfer reactions. A utilization of 2 to 3 ATP equivalents was observed for the reduction of each equivalent of NAD+. Such observations indicate that the T. neapolitanus system operated with an efficiency of approximately 80% with respect to the utilization of energy for the generation of reducing power.
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Abbreviations
- HQNO:
-
2-n-hyptyl-4-hydroxyquinoline N-oxide
- TTFA:
-
Thenoyl triflouroacetone
- CCCP:
-
m-chlorocarbonylcyanide-phenylhydrazone
- DNP:
-
2,4-dinitrophenol
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Saxena, J., Aleem, M.I.H. Generation of reducing prower in chemosynthesis. Archiv. Mikrobiol. 84, 317–326 (1972). https://doi.org/10.1007/BF00409080
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DOI: https://doi.org/10.1007/BF00409080