Summary
Scanning electron microscopy and transmission electron microscopy (carbon replicas) confirm the existence of a deep longitudinal groove on one side of the pyriform body of the zoospores of Phytophthora palmivora. Upon encystment the cell rounds off but the groove may be temporarily retained as a depression on the cyst surface. The carbon replicas revealed significant differences in outer surface texture: the zoospore surface is finely granular whereas the outer surface of both young and mature cysts are distinctly microfibrillar with only occasional patches of amorphous material.
Similar content being viewed by others
References
Bigelow, H. E., Rowley, J. R.: Surface replicas of the spores of fleshy fungi. Mycologia (N.Y.) 60, 869–887 (1968).
Bouck, G. B., Brown, D. L.: Microtubule genesis and cell shape in Ochromonas. J. Cell Biol. 47, 22a-23a (1970).
Bradley, D. E.: A study of the division of Saccharomyces cerevisiae using carbon replicas. Proceedings of the Stockholm Conference on Electron Microscopy, pp. 268–270 (1956).
Cantino, E. C., Lovett, J. S., Leak, L. V., Lythoge, J.: The single mitochondrion, fine structure and germination of the spore of Blastocladiella emersonii. J. gen. Microbiol. 31, 393–404 (1963).
Desjardins, P. R., Zentmyer, G. A., Reynolds, D. A.: Electron microscopic observations of the flagellar hairs of Phytophthora palmivora zoospores. Canad. J. Bot. 47, 1077–1079 (1969).
Fuller, M. S., Reichle, R.: The zoospore and early development of Rhizidiomyces apophysatus. Mycologia (N.Y.) 57, 946–961 (1965).
Fultz, S., Woolf, R. A.: Surface structure in Allomyces during germination and growth. Mycologia (N. Y.) 64, 212–213 (1974).
Grove, S., Bracker, C. E.: Personal communication (1971).
Heath, I. B., Greenwood, A. D.: Wall formation in the Saprolegniales. Arch. Mikrobiol. 75, 67–79 (1970).
Hill, E. P.: The fine structure of the zoospores and cysts of Allomyces macrogynus. J. gen. Microbiol. 56, 125–130 (1969).
Ho, H. H., Hickman, C. J., Telford, R. W.: The morphology of zoospores of Phytophthora megasperma var. sojae and other Phycomycetes. Canad. J. Bot. 46, 88–89 (1968a).
Ho, H. H., Zachariah, K., Hickman, C. J.: The ultrastructure of zoospores of Phytophthora megasperma var. sojae. Canad. J. Bot. 46, 37–41 (1968b).
Reichle, R. E.: Fine structure of Phytophthora parasitica zoospores. Mycologia (N. Y.) 61, 30–51 (1969a).
Reichle, R. E.: Retraction of flagella by Phytophthora parasitica var. nicotiana zoospores. Arch. Mikrobiol. 66, 340–347 (1969b).
Rowley, J. R., Flynn, J. J.: Single-stage carbon replica of microspores. Stain Technol. 41, 287–290 (1966)
Silveira, M., Porter, K. R.: The spermatozoids of flatworms and their microtubular systems. Protoplasma (Wien) 59, 240–265 (1964).
Sing, V. O., Bartnicki-Garcia, S.: Adhesion of zoospores of Phytophthora palmivora to solid surfaces. Phytopathology 62, 790 (1972).
Tokunaga, J., Bartnicki-Garcia, S.: Cyst wall formation and endogenous carbohydrate utilization during synchronous encystment of Phytophthora palmivora zoospores. Arch. Mikrobiol. 79, 283–292 (1971a).
Tokunaga, J., Bartnicki-Garcia, S.: Structure and differentiation of the cell wall of Phytophthora palmivora; cysts, hyphae and sporangia. Arch. Mikrobiol. 79, 293–310 (1971b).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Desjardins, P.R., Wang, M.C. & Bartnicki-Garcia, S. Electron microscopy of zoospores and cysts of Phytophthora palmivora: Morphology and surface texture. Archiv. Mikrobiol. 88, 61–70 (1973). https://doi.org/10.1007/BF00408841
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00408841