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Decreased expression of cardiac sarcoplasmic reticulum Ca2+-pump ATPase in congestive heart failure due to myocardial infarction

  • Part II: Cardiac Hypertrophy and Failure
  • Published:
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Abstract

Myocardial infarction in rats induced by occluding the left coronary artery for 4, 8 and 16 weeks has been shown to result in congestive heart failure (CHF) characterized by hypertrophy of the viable ventricular myocardial tissue. We have previously demonstrated a decreased calcium transport activity in the sarcoplasmic reticulum (SR) of post-myocardial infarction failing rat hearts. In this study we have measured the steady state levels of the cardiac SR Ca2+-pump ATPase (SERCA2) mRNA using Northern blot and slot blot analyses. The relative amounts of SERCA2 mRNA were decreased with respect to GAPDH mRNA and 28 S rRNA in experimental failing hearts at 4 and 8 weeks post myocardial infarction by about 20% whereas those at 16 weeks declined by about 35% of control values. The results obtained by Western blot analysis, revealed that the immunodetectable levels of SERCA2 protein in 8 and 16 weeks postinfarcted animals were decreased by about 20% and 30%, respectively. The left ventricular SR Ca2+-pump ATPase specific activity was depressed in the SR preparations of failing hearts as early as 4 weeks post myocardial infarction and declined by about 65% at 16 weeks compared to control. These results indicate that the depressed SR Ca2+-pump ATPase activity in CHF may partly be due to decreased steady state amounts of SERCA2 mRNA and SERCA2 protein in the failing myocardium.

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Authors and Affiliations

  1. Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada

    Angel Zarain-Herzberg, Nasir Afzal, Vijayan Elimban & Naranjan S. Dhalla

  2. Department of Physiology, Faculty of Medicine, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada

    Angel Zarain-Herzberg, Nasir Afzal, Vijayan Elimban & Naranjan S. Dhalla

Authors
  1. Angel Zarain-Herzberg
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  2. Nasir Afzal
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  3. Vijayan Elimban
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  4. Naranjan S. Dhalla
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Zarain-Herzberg, A., Afzal, N., Elimban, V. et al. Decreased expression of cardiac sarcoplasmic reticulum Ca2+-pump ATPase in congestive heart failure due to myocardial infarction. Mol Cell Biochem 163, 285–290 (1996). https://doi.org/10.1007/BF00408669

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