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The phenoloxidases of the Ascomycete Podospora anserina

VII. Quantitative changes in the spectrum of phenoloxidases during growth in submerged culture

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Summary

In order to learn the internal conditions for the production of the various phenoloxidases produced by the Ascomycete Podospora anserina the wild strain has been grown under controlled conditions in a fermenter for a period of 34 days. Samples were withdrawn at regular intervals and assayed for mycelial yield and intra- and extracellular phenoloxidase production.

Maximal yield was obtained at the following age of the culture: Mycelial production 9 d, tyrosinase 4 d, the high molecular weight laccase I between 9 and 19 d. The low molecular weight laccases II and III, initially present in medium concentrations, dropped to an early minimum after 4 days, followed by an increase with a maximum in the late autolytic phase.

The changes in the phenoloxidase spectrum and the antiparallel production curve for the high molecular weight against the low molecular weight laccases are discussed in relation to the earlier observed genetical and physiological control of phenoloxidase synthesis and in relation to the possibility of laccase I being composed of active subunits of low molecular weight laccases.

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References

  • Die Phenoloxydasen des Ascomyceten Podospora anserina. Part I. Die Identifizierung von Laccase und Tyrosinase beim Wildstamm. Arch. Mikrobiol. 46, 217–226 (1963) (K. Esser).

    PubMed  Google Scholar 

  • Die Phenoloxydasen des Ascomyceten Podospora anserina. Part II. Reinigung und Eigenschaften der Laccase. Arch. Mikrobiol. 48, 306–318 (1964) (K. Esser, S. Dick u. W. Gielen).

    PubMed  Google Scholar 

  • Die Phenoloxydasen des Ascomyceten Podospora anserina. Part III. Quantitative und qualitative Enzymunterschiede nach Mutation an nicht gekoppelten Loci. Z. Vererbungsl. 97, 327–344 (1966) (K. Esser).

    PubMed  Google Scholar 

  • Die Phenoloxydasen des Ascomyceten Podospora anserina. Part IV. Reinigung und Eigenschaften der Tyrosinase. Arch. Mikrobiol. 65, 146–162 (1969) (F. Herzfeld u. K. Esser).

    PubMed  Google Scholar 

  • Die Phenoloxydasen des Ascomyceten Podospora anserina. Part V. Eigenschaften der Laccase I nach weiterer Reinigung. Arch. Mikrobiol. 72, 267–296 (1970) (H. P. Molitoris u. K. Esser).

    PubMed  Google Scholar 

  • Die Phenoloxydasen des Ascomyceten Podospora anserina. Part VI. Genetic regulation of the formation of laccase. Genetics 64, 441–458 (1970) (K. Esser and W. Minuth).

    PubMed  Google Scholar 

  • Bocks, S. M.: Fungal metabolism. II. Studies on the formation and activity of p-diphenol oxidase (laccase). Phytochemistry 6, 777–783 (1967).

    Article  Google Scholar 

  • Burnett, J. B., Holstein, T. J., Quevedo, W. C., Jr.: Electrophoretic variations of tyrosinase in follicular melanocytes during the hair growth cycle in mice. J. exp. Zool. 171, 369–376 (1969).

    PubMed  Google Scholar 

  • Černá, J., Musílek, V.: Enzymatic activity in basidiomycetes. I. Submerged growth and oxidation-reduction activity of Oudemansiella mucida. Folia microbiol. (Praha) 12, 508–514 (1967).

    Google Scholar 

  • Cheung, D. S. M., Marshall, K. C.: Antigenic and some kinetic properties of three p-diphenoloxidase isoenzymes of Trametes versicolor. Biochim. biophys. Acta (Amst.) 178, 177–180 (1969).

    Google Scholar 

  • Esser, K.: Wachstum, Fruchtkörper- und Pigmentbildung von Podospora anserina in synthetischen Nährmedien. C. R. Lab. Carlsberg, Sér. Physiol. 26, 103–116 (1956a).

    Google Scholar 

  • —: Die Incompatibilitätsbeziehungen zwischen geographischen Rassen von Podospora anserina (CES.) Rehm. I. Genetische Analyse der Semi-Incompatibilität. Z. indukt. Abstamm- u. Vereb.-L. 87, 595–624 (1956b).

    Article  Google Scholar 

  • —: Die Incompatibilitätsbeziehungen zwischen geographischen Rassen von Podospora anserina (CES.) Rehm. II. Die Wirkungsweise der Semi-Incompatibilitäts-Gene. Z. Vererbungsl. 90, 29–52 (1959).

    Google Scholar 

  • —: Die Wirkungsweise von Antiseren auf die Phenoloxydasen von Podospora anserina. Naturwissenschaften 50, 576–577 (1963).

    Google Scholar 

  • —: Phenol oxidases and morphogenesis in Podospora anserina. Genetics 60, 281–288 (1968).

    PubMed  Google Scholar 

  • —: The influence of pH on rhythmic mycelial growth in Podospora anserina. Mycologia (N. Y.) 61, 1008–1011 (1969a).

    Google Scholar 

  • —: An introduction to Podospora anserina. Neurospora Newsletter 15, 27–31 (1969b).

    Google Scholar 

  • Fåhraeus, G., Reinhammar, B.: Large scale production and purification of laccase from cultures of the fungus Polyporus versicolor and some properties of laccase A. Acta chem. scand. 21, 2367–2378 (1967).

    PubMed  Google Scholar 

  • Fox, A. S., Burnett, J. B., Fuchs, M. S.: Tyrosine as a model for the genetic control of protein synthesis. Ann. N. Y. Acad. Sci. 100, Part II, 840–856 (1963).

    PubMed  Google Scholar 

  • Grabbe, K., Koenig, R., Haider, K.: Die Bildung der Phenoloxydase und die Stoffwechselbeeinflussung durch Phenole bei Polystictus versicolor. Arch. Mikrobiol. 63, 133–153 (1968).

    PubMed  Google Scholar 

  • Lyr, H.: Über den Nachweis von Oxydasen und Peroxydasen bei höheren Pilzen und die Bedeutung dieser Enzyme für die Bavendamm-Reaktion. Planta (Berl.) 50, 359–370 (1958).

    Google Scholar 

  • —: Alterung und Enzymbildung bei höheren Pilzen. Z. allg. Mikrobiol. 4, 249–258 (1964).

    Google Scholar 

  • Lysek, G., Esser, K.: Rhythmic mycelial growth in Podospora anserina. I. The pleiotropic phenotype of a mutant is caused by a point mutation. Arch. Mikrobiol. 73, 224–230 (1970).

    Google Scholar 

  • ——: Rhythmic mycelial growth in Podospora anserina. II. Evidence for a correlation with carbohydrate metabolism. Arch. Mikrobiol. 75, 360–373 (1971).

    Google Scholar 

  • Malmström, G., Fåhraeus, G., Mosbach, R.: Purification of laccase. Biochim. biophys. Acta (Amst.) 28, 652–653 (1958).

    Article  Google Scholar 

  • Stafford, H. A.: Changes in phenolic compounds and related enzymes in young plants of Sorghum. Phytochemistry 8, 743–752 (1969).

    Article  Google Scholar 

  • Warburg, O., Christian, W.: Isolierung und Kristallisation des Gärungsfermentes Enolase. Biochem. Z. 310, 384–421 (1941)

    Google Scholar 

  • Wrigley, C.: Gel electrofocusing—a technique for analyzing multiple protein samples by isoelectric focusing. Sci. Tools 15, 17–23 (1968).

    Google Scholar 

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Authors and Affiliations

  1. Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Germany

    H. Peter Molitoris & Karl Esser

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  1. H. Peter Molitoris
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  2. Karl Esser
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With support of the Deutsche Forschungsgemeinschaft, Bad Godesberg (Germany).

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Molitoris, H.P., Esser, K. The phenoloxidases of the Ascomycete Podospora anserina . Archiv. Mikrobiol. 77, 99–110 (1971). https://doi.org/10.1007/BF00408602

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  • DOI: https://doi.org/10.1007/BF00408602

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