Abstract
Small cryptic plasmids of molecular weights ranging from 1 to 3 Mdal were detected by electron microscopy in Salmonella typhimurium strain LT2 (ColIb). They were divided into different size classes. Two of the cryptic plasmids were transferred simultaneously with ColIb to Escherichia coli.
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Abbreviations
- TES buffer:
-
0.05 M tris-HCl pH 8.0, 0.05 M NaCl, 0.005 M Na2 EDTA
- TCG medium:
-
tris-casamino acid-glucose medium
- cpm:
-
counts per min
References
Adler, H. J., Fisher, W. P., Cohen, A., Hardigree, A. A.: Miniature Escherichia coli cells deficient in DNA. Proc. Natl. Acad. Sci. U.S.A. 57, 321–326 (1967)
Bollum, F. J.: Filter paper disc techniques for assaying radioactive macromolecules, In: Methods in enzymology, Vol. 12B (L. Grossman, K. Moldave, eds.), pp. 169–173. New York: Academic Press 1968
Clewell, D. B., Helinski, D. R.: Supercoiled circular DNA protein complex in Escherichia coli. Purification and induced conversion to an open circular DNA form. Proc. Natl. Acad. Sci. U.S.A. 62, 1159–1166 (1969)
Clewell, D. B., Helinski, D. R.: Existence of the colicinogenic factor-sex factor ColIb-P9 as a supercoiled circular DNA protein relaxation complex. Biochem. Biophys. Res. Commun. 41, 150–156 (1970)
Clowes, R. C.: Molecular structure of bacterial plasmids. Bacteriol. Rev. 36, 361–405 (1972)
Cozarelli, N., Kelly, R., Kornberg, A.: A minute circular DNA from E. coli 15. Proc. Natl. Acad. Sci. U.S.A. 60, 992–999 (1968)
Dowman, J. E., Meynell, G. G.: Pleiotropic effects of derepressed bacterial sex-factors on colicinogeny and cell wall structure. Molec. Gen. Genet. 109, 57–68 (1970)
Goebel, W., Schrempf, H.: Isolation of minicircular deoxyribonucleic acid from wild strains of Escherichia coli and their relationship to other bacterial plasmids. J. Bact. 111, 696–704 (1972)
Harkness, N. K., Murray, M. L.: Occurence of cryptic plasmids in Haemophilus influenzae. FEMS Microbiol. Lett. 1, 239–241 (1977)
Ikeda, H., Inuzuka, M., Tomizawa, Y.: P-1 like plasmid in Escherichia coli 15. J. Mol. Biol. 15, 457–470 (1970)
Inselburg, J.: Segregation into and replication of plasmid deoxyribonucleic acid in chromosomeless segregants of Escherichia coli. J. Bact. 102, 642–647 (1970)
Kleinschmidt, A. K.: Monolayer Techniques in electron microscopy of nucleic acids molecules. In: Methods in enzymology, Vol. 12B (L. Grossman, K. Moldave, eds.), pp. 361–377. New York: Academic Press 1968
Kozinski, A. W., Szybalski, W.: Dispersive transfer of the parental DNA molecule to the progeny of phage ϕX-174. Virology 9, 260–274 (1959)
Porter, B. W., Kolodner, R., Warner, R. C.: Plasmids of Shigella dysenteriae Y6R: a defective Col factor. J. Bact. 116, 163–174 (1973)
Rhoades, M., MacHattie, L. A., Thomas, C. A., Jr.: The P22 bacteriophage DNA molecule. I. The mature form. J. Mol. Biol. 37, 21–40 (1968)
Sheehy, R. J., Allison, D. P., Curtiss, R. III: Cryptic plasmids in minicells-producing strains of Salmonella typhimurium. J. Bact. 114, 439–442 (1973)
Smith, H. R., Humphreys, G. O., Grindley, N. D. F., Grindley, J. N., Anderson, E. S.: Molecular studies of an fi+ plasmid from strains of Salmonella typhimurium. Molec. Gen. Genet. 126, 143–151 (1973)
Spratt, B. G., Rowbury, R. J., Meynell, G. G.: The plasmid of Salmonella typhimurium LT2. Molec. Gen. Genet. 121, 347–353 (1973)
Tanaka, T., Koshikawa, T.: Isolation and characterization of four types of plasmids from Bacillus subtilis (natto). J. Bact. 131, 699–701 (1977)
Zandvliet, G. M., Jansz, H. S.: Characterization and replication control of plasmids from Enterobacter cloacae DF13. Eur. J. Biochem. 62, 439–449 (1976)
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Deryło, M., Skorupska, A. & Lorkiewicz, Z. Detection of small cryptic plasmids in Salmonella typhimurium strain LT2 . Arch. Microbiol. 119, 87–90 (1978). https://doi.org/10.1007/BF00407933
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DOI: https://doi.org/10.1007/BF00407933