Skip to main content
Log in

Detection of small cryptic plasmids in Salmonella typhimurium strain LT2

  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Small cryptic plasmids of molecular weights ranging from 1 to 3 Mdal were detected by electron microscopy in Salmonella typhimurium strain LT2 (ColIb). They were divided into different size classes. Two of the cryptic plasmids were transferred simultaneously with ColIb to Escherichia coli.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

TES buffer:

0.05 M tris-HCl pH 8.0, 0.05 M NaCl, 0.005 M Na2 EDTA

TCG medium:

tris-casamino acid-glucose medium

cpm:

counts per min

References

  • Adler, H. J., Fisher, W. P., Cohen, A., Hardigree, A. A.: Miniature Escherichia coli cells deficient in DNA. Proc. Natl. Acad. Sci. U.S.A. 57, 321–326 (1967)

    Google Scholar 

  • Bollum, F. J.: Filter paper disc techniques for assaying radioactive macromolecules, In: Methods in enzymology, Vol. 12B (L. Grossman, K. Moldave, eds.), pp. 169–173. New York: Academic Press 1968

    Google Scholar 

  • Clewell, D. B., Helinski, D. R.: Supercoiled circular DNA protein complex in Escherichia coli. Purification and induced conversion to an open circular DNA form. Proc. Natl. Acad. Sci. U.S.A. 62, 1159–1166 (1969)

    PubMed  Google Scholar 

  • Clewell, D. B., Helinski, D. R.: Existence of the colicinogenic factor-sex factor ColIb-P9 as a supercoiled circular DNA protein relaxation complex. Biochem. Biophys. Res. Commun. 41, 150–156 (1970)

    PubMed  Google Scholar 

  • Clowes, R. C.: Molecular structure of bacterial plasmids. Bacteriol. Rev. 36, 361–405 (1972)

    PubMed  Google Scholar 

  • Cozarelli, N., Kelly, R., Kornberg, A.: A minute circular DNA from E. coli 15. Proc. Natl. Acad. Sci. U.S.A. 60, 992–999 (1968)

    PubMed  Google Scholar 

  • Dowman, J. E., Meynell, G. G.: Pleiotropic effects of derepressed bacterial sex-factors on colicinogeny and cell wall structure. Molec. Gen. Genet. 109, 57–68 (1970)

    PubMed  Google Scholar 

  • Goebel, W., Schrempf, H.: Isolation of minicircular deoxyribonucleic acid from wild strains of Escherichia coli and their relationship to other bacterial plasmids. J. Bact. 111, 696–704 (1972)

    PubMed  Google Scholar 

  • Harkness, N. K., Murray, M. L.: Occurence of cryptic plasmids in Haemophilus influenzae. FEMS Microbiol. Lett. 1, 239–241 (1977)

    Google Scholar 

  • Ikeda, H., Inuzuka, M., Tomizawa, Y.: P-1 like plasmid in Escherichia coli 15. J. Mol. Biol. 15, 457–470 (1970)

    Google Scholar 

  • Inselburg, J.: Segregation into and replication of plasmid deoxyribonucleic acid in chromosomeless segregants of Escherichia coli. J. Bact. 102, 642–647 (1970)

    PubMed  Google Scholar 

  • Kleinschmidt, A. K.: Monolayer Techniques in electron microscopy of nucleic acids molecules. In: Methods in enzymology, Vol. 12B (L. Grossman, K. Moldave, eds.), pp. 361–377. New York: Academic Press 1968

    Google Scholar 

  • Kozinski, A. W., Szybalski, W.: Dispersive transfer of the parental DNA molecule to the progeny of phage ϕX-174. Virology 9, 260–274 (1959)

    PubMed  Google Scholar 

  • Porter, B. W., Kolodner, R., Warner, R. C.: Plasmids of Shigella dysenteriae Y6R: a defective Col factor. J. Bact. 116, 163–174 (1973)

    PubMed  Google Scholar 

  • Rhoades, M., MacHattie, L. A., Thomas, C. A., Jr.: The P22 bacteriophage DNA molecule. I. The mature form. J. Mol. Biol. 37, 21–40 (1968)

    PubMed  Google Scholar 

  • Sheehy, R. J., Allison, D. P., Curtiss, R. III: Cryptic plasmids in minicells-producing strains of Salmonella typhimurium. J. Bact. 114, 439–442 (1973)

    PubMed  Google Scholar 

  • Smith, H. R., Humphreys, G. O., Grindley, N. D. F., Grindley, J. N., Anderson, E. S.: Molecular studies of an fi+ plasmid from strains of Salmonella typhimurium. Molec. Gen. Genet. 126, 143–151 (1973)

    PubMed  Google Scholar 

  • Spratt, B. G., Rowbury, R. J., Meynell, G. G.: The plasmid of Salmonella typhimurium LT2. Molec. Gen. Genet. 121, 347–353 (1973)

    PubMed  Google Scholar 

  • Tanaka, T., Koshikawa, T.: Isolation and characterization of four types of plasmids from Bacillus subtilis (natto). J. Bact. 131, 699–701 (1977)

    PubMed  Google Scholar 

  • Zandvliet, G. M., Jansz, H. S.: Characterization and replication control of plasmids from Enterobacter cloacae DF13. Eur. J. Biochem. 62, 439–449 (1976)

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Deryło, M., Skorupska, A. & Lorkiewicz, Z. Detection of small cryptic plasmids in Salmonella typhimurium strain LT2 . Arch. Microbiol. 119, 87–90 (1978). https://doi.org/10.1007/BF00407933

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00407933

Key words

Navigation