Abstract
The complements of restriction endonucleases of 12 strains of cyanobacteria were determined in cell-free extracts, and were compared with the complements of restriction activities assessed by measuring the relative efficiencies of plating of cyanophages on those cyanobacteria. The hosts which were susceptible to all of the phages contained endo R · AvaI and endo R · AvaII, and in several cases probably endo R · AvaIII, or isoschizomers of these enzymes. Three hosts which were lysed by only a subset (1 or 3) of the phages contained different restriction endonuclease. Anabaena sp. PCC 7120 showed apparent phenotypic restriction of phage An-22 grown in hosts with (isoschizomers of) AvaI, II and III, but no corresponding endonuclease has yet been detected in vitro. Nostoc sp. ATCC 29131 (PCC 6705) was found to contain a restriction enzyme, NspBII, with hitherot unknown specificity, C(A/C)GC(T/G)G.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allen MB, Arnon DI (1955) Studies on nitrogen-fixing blue-green algae I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiol 30:366–372
Arber W, Linn S (1969) DNA modification and restriction. Ann Rev Biochem 38:467–500
Bernards R, Houweling A, Schrier PI, Bos JL, Van der Eb AJ (1982) Characterization of cells transformed by Ad5/Ad12 hybrid early region I plasmids. Virology 120:422–432
Buchman AR, Burnett L, Berg P (1981) The SV 40 nucleotide sequence. In: J Tooze (ed) Molecular biology of tumor viruses, 2nd ed.: DNA Tumor viruses (Revised) Cold Spring Harbor, pp 799–841
Currier TC, Wolk CP (1979) Characteristics of Anabaena variabilis influencing plaque formation by cyanophage N-1. J Bacteriol 139:88–92
Duyvesteyn M, de Waard A (1980) A new sequence specific endonuclease from a thermophilic cyanobacterium Mastigocladus laminosus. FEBS Lett 111:423–426
Hu N-T, Thiel T, Giddings TH Jr, Wolk CP (1981) New Anabaena and Nostoc cyanophages from sewage settling ponds. Virology 114:236–246
Hughes SG, Murray K (1980) The nucleotide sequences recognized by endonucleases AvaI and AvaII from Anabaena variabilis. Biochem J 185:65–75
Krüger DH, Hansen S, Schroeder C, Presber W (1977) Host-dependent modification of bacteriophage T7 and SAM ase negative T3-derivatives affecting their absorption ability. Molec Gen Genet 153:107–110
Krüger, DH, Hansen S, Schroeder C (1980) Host-dependent modification of bacterial virus T3 affecting its adsorption ability. Virology 102:444–466
Lupker HSC, Dekker BMM (1981) Purification of the sequence-specific endonuclease SinI from Salmonella infantis. Biochim Biophys Acta 654:297–299
Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with basespecific chemical cleavages. In: Grossman L, Moldave K (eds) Methods in Enzymology, vol 65. Academic Press New York, pp 499–560
Murray K, Hughes SG, Brown JS, Bruce SA (1976) Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis. Biochem J 159:317–322
Reaston J, Duyvesteyn MGC, de Waard A (1982) Nostoc PCC7524, a cyanobacterium which contains five sequence-specific deoxyribonucleases. Gene 20:103–110
Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier R (1979) Generic assignment, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61
Roberts RJ (1983) Restriction and modification enzymes and their recognition sequences. Nucleic Acid Res 11:r135-r167
Roizes G, Nardeux P-C, Monier R (1979) A new specific endonuclease from Anabaena variabilis. FEBS Lett 104:39–44
Sanger F, Coulson AR, Friedman T, Air GM, Barrell BG, Brown N, Fiddes JC, Hutschison III CA, Slocombe PM, Smith M (1978) The nucleotide sequence of bacteriophage ØX174. J Mol Biol 125:225–246
Sutcliffe JG (1978) Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp Quant Biol USA 43:77–90
Sutcliffe JG, Church GM (1978) The cleavage site of the restriction endonuclease AvaII. Nucleic Acids Res 5:2313–2319
Tu CD, Wu R (1980) Sequence analysis of short DNA fragments. In: Grossman L, Moldave K (eds) Methods in Enzymology, vol 65, Academic Press, New York, pp 620–638
Van den Hondel CAMJJ, Van Leen RW, Van Arkel GA, Duyvesteyn MGC, de Waard A (1983) Sequence-specific nucleases from the cyanobacterium Fremyella diplosiphon, and a peculiar resistance of its chromosomal DNA towards cleavage by other restriction enzymes. FEMS Microbiol Lett 16:7–12
Waard A de, Duyvesteyn MGC (1980) Are sequence-specific deoxyribonucleases of value as taxonomic markers of cyanobacterial species? Arch Microbiol 128:242–247
Waard A de, Korsuize J, Van Beveren CP, Maat J (1978) A new sequence-specific endonuclease from Anabaena cylindrica. FEBS Lett 96: 106–110
Waard A de, Van Beveren CP, Duyvesteyn MGC, Van Ormondt H (1979) Two sequence-specific endonucleases from Anabaena oscillarioides. FEBS Lett 101:71–76
Wezenbeek PMGF Van, Hulsebos ThJM, Schoenmakers JJG (1980) Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene 11:129–148
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Duyvesteyn, M.G.C., Korsuize, J., de Waard, A. et al. Sequence-specific endonucleases in strains of Anabaena and Nostoc . Arch. Microbiol. 134, 276–281 (1983). https://doi.org/10.1007/BF00407802
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00407802