Abstract
Recently, some evidence for the occurence of a light-independent protochlorophyllide-reducing enzyme in greening barley plants has been presented. In the present work this problem was reinvestigated. δ-[14C] Aminolevulinic acid was fed to isolated barley shoots from plants which had been preilluminated for various lengths of time. Porphyrins which had been synthesized during the dark incubation were analyzed by high-performance liquid chromatography. There was no evidence for a light-independent synthesis of chlorophyll(ide). The 14C-labelled precursor was incorporated almost exclusively into protochlorophyllide. The reduction of labelled protochlorophyllide to chlorophyllide was strictly light-dependent. These results are not consistent with the existence of a light-independent protochlorophyllide-reductase in barley as proposed previously.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- HPLC:
-
high-performance liquid chromatography
References
Adamson, H. (1983) Evidence for a light-independent protochlorophyllide reductase in green barley leaves. In: Cell function and differentiation 2, pp. 33–41, Akoyunoglou, G., Evangelopoilos, A.E., Georgtsos, J., Palaiologos, G., Trakatellis, A., Tsiganos, C.P., eds. Proc. Spec. FEBS Meet., Athens, 1982, Liss, New York
Adamson, H., Hiller, R.G., Vesk, M. (1980) Chloroplast development and the synthesis of chlorophyll a and b and chlorophyll protein complexes I and II in the dark in Tradescantia albiflora (Knuth). Planta 150, 269–274
Adamson, H., Packer, N., Sanders, N. (1983) Chlorophyll synthesis in the dark in peas. 6th Int. Congr. Photosynthesis, Brussels, Book of Abstracts, vol. 2, p. 242
Apel, K. (1981) The protochlorophyllide holochrome of barley (Hordeun vulgare L.). Phytochrome-induced decrease of translatable mRNA coding for the NADPH-protochlorophyllide oxidoreductase. Eur. J. Biochem. 120, 89–93
Apel, K., Santel, H.-J., Redlinger, T.E., Falk, H. (1980) The protochlorophyllide holochrome of barley (Hordeum vulgare L.). Isolation and characterization of the NADPH protochlorophyllide oxidoreductase. Eur. J. Biochem. 111, 251–258
Bennett, J. (1981) Biosynthesis of the light-harvesting chlorophyll a/b protein. Polypeptide turnover in darkness. Eur. J. Biochem. 118, 61–70
Boardman, N.K. (1966) Protochlorophyll. In: The chlorophylls, pp. 437–476, Vernon, L.P., Seely, G.R., eds. Academic Press, New York London
Boardman, N.K., Anderson, J.M., Goodchild, D.J. (1978) Chlorophyll-protein complexes and structure of mature and developing chloroplasts. Curr. Top. Bioenerg. 8, 35–109
Boffey, S.A., Sellden, G., Leech, R.M. (1980) The influence of cell age on chlorophyll formation in light-grown and etiolated wheat seedlings. Plant Physiol 65, 680–684
Bogorad, L. (1976) Chlorophyll biosynthesis. In: Chemistry and biochemistry of plant pigments, 2nd edn., vol. 1, pp. 64–148, Goodwin, T.W., ed. Academic Press, New York London
Castelfranco, P.A., Beale, S.T. (1983) Chlorophyll biosynthesis: recent advances and areas of current interest. Annu. Rev. Plant Physiol. 34, 241–278
Dehesh, K., Häuser, I., Apel, K., Kloppstech, K. (1983) The distribution of NADPH-protochlorophyllide oxidoreductase in relation to chlorophyll accumulation along the barley leaf gradient. Planta 158, 134–139
Eskins, K., Harris, L. (1981) High-performance liquid chromatography of etioplast pigments in red kidney bean leaves. Photochem. Photobiol. 33, 131–133
Griffiths, W.T. (1974) Protochlorophyll and protochlorophyllide as precursors for chlorophyll synthesis in vitro. FEBS Lett. 49, 196–200
Griffiths, W.T. (1978) Reconstitution of chlorophyllide formation by isolated etioplast membranes. Biochem. J. 174, 681–692
Häuser, I., Dehesh, K., Apel, K. (1984) The proteolytic degradation in vitro of the NADPH-protochlorophyllide oxidoreductase of barley (Hordeum vulgare L.). Arch. Biochem. Biophys. 228, 577–586
Houssier, C., Sauer, K. (1969) Optical properties of the protochlorophyll pigments. I. Isolation, characterization, and infrared spectra. Biochim. Biophys. Acta 172, 476–491
Ikeuchi, M., Murakami, S. (1982) Behavior of the 36000 dalton protein in the internal membranes of squash etioplasts during greening. Plant Cell Physiol. 23, 575–583
Kay, S.A., Griffiths, W.T. (1983) Light-induced breakdown of NADPH-protochlorophyllide oxidoreductase in vitro. Plant Physiol. 72, 229–236
Kirk, J.T.O., Tilney-Bassett, R.A.E. (1978) The plastids. Freeman, London San Francisco
Mapleston, E.R., Griffiths, W.T. (1980) Light modulation of the activity of protochlorophyllide reductase. Biochem. J. 189, 125–133
Meyer, G., Bliedung, H., Kloppstech, K. (1983) NADPH-protochlorophyllide oxidoreductase: reciprocal regulation in mono- and dicotyledonean plants. Plant Cell Reports 2, 26–29
Redlinger, T.E., Apel, K. (1980) The effect of light on four protochlorophyllide-binding polypeptides of barley (Hordeum vulgare). Arch. Biochem. Biophys. 200, 253–260
Robertson, D., Laetsch, M. (1974) Structure and function of developing barley plastids. Plant Physiol. 54, 148–159
Santel, H.J., Apel, K. (1981) The protochlorophyllide holochrome of barley (Hordeum vulgare L.). The effect of light on the NADPH: protochlorophyllide oxidoreductase. Eur. J. Biochem. 120, 95–103
Schopfer, P., Apel, K. (1983) Intracellular photomorphogenesis. In: Encyclopedia of plant physiology, N.S., vol. 16: Photomorphogenesis, pp. 258–288, Shropshire, W., Jr., Mohr, H., eds. Springer, Berlin Heidelberg New York
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Apel, K., Motzkus, M. & Dehesh, K. The biosynthesis of chlorophyll in greening barley (Hordeum vulgare). Is there a light-independent protochlorophyllide reductase?. Planta 161, 550–554 (1984). https://doi.org/10.1007/BF00407088
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00407088