Summary
The anaerobic glucose uptake (at 20°, pH 3.5) by resting cells of Saccharomyces cerevisiae followed unidirectional Michaelis-Menten kinetics and was competitively inhibited by l-sorbose; K m and K i were respectively 5.6×10-4 m and 1.8×10-1 m; V max was 6.5×10-8 moles mg-1 min-1. The aerobic uptake of glucose by resting yeast was also inhibited by l-sorbose but did not follow unidirectional Michaelis-Menten kinetics. Glucose-limited growth in the chemostat of a respiration-deficient mutant of S. cerevisiae was competitively inhibited by l-sorbose. As predicted by theory for transport-limited growth in the chemostat (van Uden, 1967) the steady state glucose concentrations were linear functions of the l-sorbose concentrations with different slopes at different dilution rates; K m and K i were respectively 7.2×10-4 m and 1.8×10-1 m. It is concluded that glucose transport was the rate-limiting step of anaerobic fermentation of S. cerevisiae and of growth of the mutant and that l-sorbose is a competitive inhibitor of active glucose transport in this yeast. The latter conclusion is accommodated in the transport model of van Steveninck and Rothstein (1965).
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van Uden, N. Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition. Archiv. Mikrobiol. 58, 155–168 (1967). https://doi.org/10.1007/BF00406676
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DOI: https://doi.org/10.1007/BF00406676