Summary
A large number of cytochemical staining techniques together with chemical and enzymic extractions have been used on Prorocentrum micans. The object of this study being to ascertain the main chemical components of the unusual chromosomes found in the Dinophyceae.
DNA was the only major component which could be positively determined and unlike most other chromosomes these appeared to contain no protein or RNA. However, the chromosomes were embedded in a granular proteinaceous “nuclear matrix,” the function of which is as yet unknown. The similarity in the composition and structure of the chromosomes of Prorocentrum and the nuclei of Bacteria are discussed.
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Alfert, M., and I. I. Geschwind: A selective staining method for the basic proteins of cell nuclei. Proc. nat. Acad. Sci. (Wash.) 39, 991–999 (1953).
Bloch, D. P., and H. Y. C. Hew: Schedule of spermatogenesis in the pulmonate snail Helix aspersa with special reference to histone transition. J. biophys. biochem. Cytol. 7, 515–532 (1960).
Brachet, J.: Biochemical Cytology. New York: Academic Press 1957.
Dodge, J. D.: The nucleus and nuclear division in the Dinophyceae. Arch. Protistenk. 106, 442–452 (1963a).
—: Chromosome structure in the Dinophyceae. I. The spiral chromonema. Arch. Mikrobiol. 45, 46–57 (1963b).
Giesbrecht, P.: Vergleichende Untersuchungen über einige Reaktionen der Chromosomen von Bacillus megaterium und Amphidinium elegans. 4th Int. Kongr. E. M. Berlin (1958), S. 251–255 (1960).
—: Vergleichende Untersuchungen an den Chromosomen des Dinoflagellaten Amphidinium elegans und denen der Bakterien. Zbl. Bakt., I. Abt. Orig. 187, 452–498 (1962).
Grell, K., and K. E. Wohlfarth-Bottermann: Licht- und elektronenmikroskopische Untersuchungen an dem Dinoflagellaten Amphidinium elegans n.sp. Z. Zellforsch. 47, 7–17 (1957).
Jensen, W. A.: Botanical Histochemistry. San Francisco: Freeman 1962.
Kellenberger, E., A. Ryter, and J. Séchaud: Electron microscope study of DNA-containing plasms II. J. biophys. biochem. Cytol. 4, 671–678 (1958).
—: The physical state of the bacterial nucleus. In: Microbial Genetics. p. 39–66. London: Cambridge University Press 1960.
—: The study of natural and artificial DNA-plasms by thin sections. Symp. Int. Soc. Cell Biol. 1, 233–249 (1962).
Korson, R.: A differential stain for nucleic acids. Stain Technol. 26, 265–270 (1951).
Kran, K.: Die Struktur des Chromosoms von Escherichia coli. Arch. Mikrobiol. 44, 1–22 (1962).
La Cour, L. F., and J. Chayen: A cyclic staining behaviour of the chromosomes during mitosis and meisosis. Exp. Cell Res. 14, 462–468 (1958).
Mazia, D., P. A. Breuer, and M. Alfert: The cytochemical staining and measurement of protein with mercuric bromphenol blue. Biol. Bull. 104, 57–67 (1953).
MacGregor, H. C., and H. G. Callan: The actions of enzymes on lampbrush chromosomes. Quart. J. mier. Sci. 103, 173–203 (1962).
Ris, H.: Interpretation of ultrastructure in the cell nucleus. Symp. Int. Soc. Cell Biol. 1, 69–88 (1962).
Schlote, F. W.: Über den Feinbau von Chromosomen. 1. Zur Cytologie von Escherichia coli. A. Elektronenoptische Befunde. Arch. Mikrobiol. 40, 283–305 (1961).
Serra, J. A.: Histochemical tests for proteins and amino acids; the characterization of basic proteins. Stain Technol. 21, 5–18 (1946).
Smillie, R. M., and G. Krotkov: The estimation of nucleic acids in some algae and higher plants. Canad. J. Bot. 38, 31–49 (1960).
Swift, H.: Nucleic acids and cell morphology in dipteran salivary glands. In: J. M. Allen Ed. The molecular control of cell activity. New York 1962.
Zubay, G., and M. R. Watson: The absence of histone in the bacterium Escherichia coli. I. Preparation and analysis of nucleoprotein extract. J. biophys. biochem. Cytol. 5, 51–54 (1959).
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Dodge, J.D. Chromosome structure in the dinophyceae. Archiv. Mikrobiol. 48, 66–80 (1964). https://doi.org/10.1007/BF00406598
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DOI: https://doi.org/10.1007/BF00406598