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Immunogenicity of cellular and acellular antigen preparations from a methylcholanthrene-induced mouse sarcoma

Immunogenität von zellulären und azellulären Antigen-Präparationen eines Methylcholanthren-induzierten Maus-Sarkoms

  • Original Papers
  • Experimental Oncology
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Summary

Inbred mice were immunized with various cellular and subcellular preparations from a syngeneic, methylcholanthrene-induced sarcoma to induce transplantation immunity against a subsequent challenge of viable cells from the same tumor. Only with conventional immunization procedures using radiation-attenuated tumor cells or viable tumor cells in admixture with bacillus Calmette-Guérin was transplantation immunity obtained. Neither native soluble tumor extracts prepared using several extraction methods, nor cross-linked preparations of these extracts gave rise to transplantation immunity. With few exceptions, however, the immunizing preparations resulted in the production of tumor-specific humoral antibody. The manner in which tumor antigens are presented to the host as well as the experimental system (animal strain; tumor) used appear to play an important role in the development of transplantation immunity against tumors.

Zusammenfassung

Inzuchtmäuse wurden mit verschiedenen zellulären und subzellulären Extrakten eines syngenen, Methylcholanthren-induzierten Sarkoms immunisiert, um eine Transplantationsimmunität gegen eine anschließende Implantation von lebenden Zellen des gleichen Tumors zu induzieren. Nur mit den konventionellen Immunisierungsmethoden (bestrahlte Tumorzellen oder ein Gemisch aus lebenden Tumorzellen mit Bacillus Calmette-Guérin) gelang es, eine Transplantationsimmunität zu erzielen. Weder lösliche Tumorextrakte, die durch verschiedene Extraktionsmethoden hergestellt wurden, noch die Vernetzung dieser Extrakte mit Glutaraldehyd führten zu einer Transplantationsimmunität. Mit wenigen Ausnahmen ließen sich jedoch nach Immunisierung mit diesen Extrakten tumorspezifische humorale Antikörper nachweisen. Offensichtlich scheint die Art, in der Tumorantigene dem zu immunisierenden Wirt appliziert werden, und das benutzte experimentelle Tiersystem eine entscheidende Rolle in der Entwicklung einer Transplantationsimmunität gegen Tumoren zu spielen.

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Abbreviations

BCG:

Bacillus Calmette-Guérin

ENM:

extranuclear membrane

FCA:

Freund's complete adjuvant

GA:

glutaraldehyde

PBS:

phosphate-buffered saline

NP 40:

Nonidet P 40 detergent

References

  • Avrameas S, Ternynck T (1969) The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry 6:53–66

    Google Scholar 

  • Baldwin RW, Barker CR, Embleton MJ, Glaves D, Moore M, Pimm MV (1971) Demonstration of cell-surface antigens on chemically induced tumors. Ann NY Acad Sci 177:268–278

    Google Scholar 

  • Baldwin RW (1973) Immunological aspects of chemical carcinogenesis. Adv Cancer Res 18:1–75

    Google Scholar 

  • Baldwin RW, Embleton MJ, Moore M (1973) Immunogenicity of rat hepatoma membrane fractions. Br J Cancer 28:389–399

    Google Scholar 

  • Baldwin RW, Harris JR, Price MR (1973) Fractionation of plasma membrane-associated tumor-specific antigen from an aminoazo dye-induced rat hepatoma. Int J Cancer 11:385–397

    Google Scholar 

  • Baldwin RW, Pimm MV (1973) BCG immunotherapy of a rat sarcoma. Br J Cancer 28:281–287

    Google Scholar 

  • Cantrell JL, McLaughlin CA, Ribi E (1979) Efficacy of tumor cell extracts in immunotherapy of murine EL-4 leukemia. Cancer Res 39:1159–1167

    Google Scholar 

  • DeLeo AB, Shiku H, Takahashi T, John M, Old LJ (1977) Cell surface antigens of chemically induced sarcomas of the mouse. J Exp Med 146:720–734

    Google Scholar 

  • Embleton MJ (1976) Influence of cell-free tumour-associated antigen preparations on the development of immunity to chemically induced rat tumours. Int J Cancer 18:622–629

    Google Scholar 

  • Gordon WC, Baechtel FS, Goetz G, Prager MD (1977) Immunogenicity of solubilized tumor antigen extracted from P 1798 murine lymphoma cells or isolated from tumor-bearer ascites fluid and reactivity with anti-thy-1.2 antiserum. Int J Cancer 19:692–699

    Google Scholar 

  • Herberman RB (1977) Immunogenicity of tumor antigens. Biochem Biophys Acta 473:93–119

    Google Scholar 

  • Holloway PW (1973) A simple procedure for removal of triton X-100 from protein samples. Anal Biochem 53:304–308

    Google Scholar 

  • Holmes EC, Kahan BD, Morton DL (1970) Soluble tumor-specific transplantation antigens from methylcholanthrene-induced guinea pig sarcomas. Cancer 25:373–379

    Google Scholar 

  • Law LW, Rogers MJ, Appella E (1980) Tumor antigens on neoplasms induced by chemical carcinogens and by DNA- and RNA-containing viruses: properties of the solubilized antigens. Adv Cancer Res 32:201–235

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randell RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Meltzer MS, Oppenheim JJ, Littman BH, Leonard EJ, Rapp HJ (1972) Cell-mediated tumor immunity measured in vitro and in vivo with soluble tumor-specific antigens. J Natl Cancer Inst 49:727–734

    Google Scholar 

  • Meltzer MS, Leonard EJ, Hardy AS, Rapp HJ (1975) Protective tumor immunity induced by potassium chloride extracts of guinea pig hepatomas. J Natl Cancer Inst 54:1349–1352

    Google Scholar 

  • Moore M (1978) Antigens of experimentally-induced neoplasms: a conspectus. In: Castro JE (ed) Immunological aspects of cancer. MTP Press Ltd, Lancaster

    Google Scholar 

  • Natori T, Law LW, Apella E (1977) Biological and biochemical properties of nonidet P 40-solubilized and partially purified tumor-specific antigens of the transplantation type from plasma membranes of a methylcholanthrene-induced sarcoma. Cancer Res 37:3406–3413

    Google Scholar 

  • Natori T, Law LW, Apella E (1977) Biologic and biochemical properties of detergent-solubilized tumor-specific transplantation antigen from a simian virus 40-induced neoplasm: brief communication. J Natl Cancer Inst 59:1331–1333

    Google Scholar 

  • Natori T, Law LW, Appella E (1978) Immunochemical evidence of a tumor-specific surface antigen obtained by detergent solubilization of the membranes of a chemically induced sarcoma, Meth-A. Cancer Res 38:359–364

    Google Scholar 

  • Oettgen HF, Old LJ, McLean EP, Carswell EA (1968) Delayed hypersensitivity and transplantation immunity elicited by soluble antigens of chemically induced tumours in inbred guinea pigs. Nature 220:295–297

    Google Scholar 

  • Pellis NR, Tom BH, Kahan BD (1974) Tumor-specific and allospecific immunogenicity of soluble extracts from chemically induced murine sarcomas. J Immunol 113:708–711

    Google Scholar 

  • Pellis NR, Kahan BD (1975) Specific tumor immunity induced with soluble materials: restricted range of antigen dose and of challenge tumor load for immunoprotection. J Immunol 115:1717–1722

    Google Scholar 

  • Pellis NR, Kahan BD (1976) Methods to demonstrate the immunogenicity of soluble tumor specific transplantation antigens: the immunoprophylaxis assay. Methods Cancer Res 13:291–330

    Google Scholar 

  • Pimm MV, Embleton MJ, Baldwin RW (1980) Multiple antigenic specificities within primary 3-methylcholanthrene-induced rat sarcomas and metastases. Int J Cancer 25:621–629

    Google Scholar 

  • Price MR, Baldwin RW (1974) Preparation of aminoazo dye induced rat hepatoma membrane fractions retaining tumour specific antigen. Br J Cancer 30:382–393

    Google Scholar 

  • Price MR, Baldwin RW (1977) Shedding of tumor cell surface antigens. In: Poste G, Nicolson GL (eds) Dynamic aspects of cell surface organization. Elsevier/North-Holland Biomedical Press, New York Oxford Shannon

    Google Scholar 

  • Price MR, Preston VE, Robins RA, Zöller M, Baldwin RW (1978) Induction of immunity to chemically-induced rat tumours by cellular or soluble antigens. Cancer Immunol Immunother 3:247–252

    Google Scholar 

  • Price MR, Moore VE, Baldwin RW (1979a) Biochemical aspects of tumor-specific antigens. In: Ferrone S, Gorini S, Herberman RB, Reisfeld RA (eds) Current trends in tumor immunology. Garland STPM, New York

    Google Scholar 

  • Price MR, Dennick RG, Robins RA, Baldwin RW (1979b) Modification of the immunogenicity and antigenicity of rat hepatoma cells. I. Cell-surface stabilization with glutaraldehyde. Br J Cancer 39:621–629

    Google Scholar 

  • Reisfeld RA, Kahan BD (1970) Biological and chemical characterization of human histocompatibility antigens. Fed Proc 29:2034–2040

    Google Scholar 

  • Tracey RS, Wepsic HT, Alaimo J, Morris HP (1978) Growth of transplanted rat tumors following administration of cell-free tumor antigens. Cancer Res 38:1208–1212

    Google Scholar 

  • Zöller M, Price MR, Baldwin RW (1976) Inhibition of cell-mediated cytotoxicity to chemically induced rat tumours by soluble tumour and embryo cell extracts. Int J Cancer 17:129–137

    Google Scholar 

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Authors and Affiliations

  1. Department of Internal Medicine (Cancer Research), West German Tumor Centre, University of Essen, Hufelandstr. 55, D-4300, Essen 1, Federal Republic of Germany

    K. Höffken, U. Steih & C. G. Schmidt

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  1. K. Höffken
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  2. U. Steih
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  3. C. G. Schmidt
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Supported by the Landesamt für Forschung, Ministerium für Wissenschaft und Forschung des Landes Nordrhein-Westfalen, Düsseldorf

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Höffken, K., Steih, U. & Schmidt, C.G. Immunogenicity of cellular and acellular antigen preparations from a methylcholanthrene-induced mouse sarcoma. J Cancer Res Clin Oncol 104, 237–247 (1982). https://doi.org/10.1007/BF00406244

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  • DOI: https://doi.org/10.1007/BF00406244

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