Abstract
To investigate the possible dependence of bacterial luminescence on fatty acid biosynthesis, Vibrio harveyi suspensions were treated with cerulenin. The compound caused an abrupt loss of luminescence which could be recovered by aldehyde supplementation but not with myristate supplementation. The characteristics of this cerulenin-induced light inhibition indicate that it does not result from impairment of fatty acid biosynthesis.
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References
Buttke TM, Ingram L (1978) Inhibition of unsaturated fatty acid synthesis in Escherichia coli by the antibiotic cerulenin. Biochemistry 17:5282–5286
Cline TW, Hastings JW (1974) Bacterial bioluminescence in vivo: Control and synthesis of aldehyde factor in temperature-conditional luminescence mutants. J Bacteriol 118:1059–1066
D'Agnolo G, Rosenfeld IS, Awaya J, Omura S, Vagelos PR (1973) Inhibition of fatty acid synthesis by the antibiotic cerulenin. Biochem Biophys Acta 326:155–166
Gunsalus-Miguel A, Meighen EA, Nicoli MZ, Nealson KH, Hastings JW (1972) Purification and properties of bacterial luciferases. J Biol Chem 247:398–404
Hastings JW, Nealson KH (1977) Bacterial bioluminescence. Ann Rev Microb 31:549–595
Mitchell GW, Hastings JW (1971) A stable, inexpensive, solid-state photomultiplier photometer. Anal Biochem 39:243–250
Nicoli MZ, Hastings JW (1974) Bacterial luciferase: the hydrophobic environment of the reactive sulfhydryl. J Biol Chem 249:2393–2396
Omura S (1976) The antibiotic cerulenin a novel tool for biochemistry as an inhibitor of fatty acid synthesis. Bacteriol Rev 40:681–697
Orndorff SA, Colwell RR (1980) Distribution and identification of luminous bacteria from the Sargasso Sea. Appl Environ Microb 39:983–987
Riendeau D, Meighen E (1979) Evidence for a fatty acid reductase catalyzing the synthesis of aldehydes for the bacterial bioluminescent reaction. J Biol Chem 254:7488–7490
Ulitzur S, Goldberg I (1977) Sensitive, rapid, and specific bioassay for the determination of antilipogenic compounds. Antimicrob Ag Chemoth 12:308–313
Ulitzur S, Hastings JW (1979a) Control of aldehyde synthesis in the luminous bacterium Beneckea harveyi J Bacteriol 137:854–859
Ulitzur S, Hastings JW (1979b) Evidence for tetradecanal as the natural aldehyde in bacterial luminescence. Proc Nat Acad Sci USA 76: 265–267
Ulitzur S, Hastings JW (1980) Reversible inhibition of bacterial luminescence by long-chain fatty acids. Curr Microb 3:295–300
Yetinson T, Shilo M (1979) Seasonal and geographic distribution of luminous bacteria in the eastern Mediterranean Sea and the Gulf of Elat. Appl Environ Microb 37:1230–1238
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Grogan, D.W. The response of bacterial luminescence to an inhibitor of fatty acid biosynthesis. Arch. Microbiol. 136, 137–139 (1983). https://doi.org/10.1007/BF00404788
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DOI: https://doi.org/10.1007/BF00404788