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Spontaneous autocytotoxicity against an unexpected H-2 d haplotype in MRL/lpr (H-2 k) autoimmune disease-prone mice

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Abstract

The MRL/lpr (H-2 k) inbred strain, a model for the autoimmune disease systemic lupus erythematosus, differs from the healthy inbred strain MRL +/+ (H-2 k) by only 0.1 % of its genome. Southern blot analysis using class I and class II probes confirmed the H-2 k genotype of both strains. Among the Iak-positive peritoneal cells, cells with an unexpected expression of Iad specificities were detected in a radioimmunoassay using several monoclonal antibodies and one conventional antiserum. This was only found in aged (6- to 9-month-old) mice both in the MRL/lpr strain (32 % Iad-positive mice) and in the MRL +/+ strain (42% Iad-positive mice). Furthermore, 24% of aged MRL/lpr mice exhibited strong spontaneous cytotoxic T lymphocyte (CTL) activities against P815 (H-2 d) target cells, and 57% had a weaker but still detectable level of cytotoxicity. In contrast, such a CTL activity has never been found in the MRL +/+ strain. These results suggest that the anti-H-2d d CTL plays a role in the onset of the autoimmune process in MRL/lpr mice.

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Authors and Affiliations

  1. INSERM U. 277-CNRS UA 535, Institut Pasteur, 25, rue du Docteur Roux, F-75724, Paris Cedex 15, France

    Pierre Bobé & Gabriel Gachelin

  2. INSERM U. 283, Hôpital Cochin, 27, rue du Faubourg Saint-Jacques, F-75674, Paris Cedex 14, France

    Nicole Kiger

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  1. Pierre Bobé
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  2. Gabriel Gachelin
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  3. Nicole Kiger
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Bobé, P., Gachelin, G. & Kiger, N. Spontaneous autocytotoxicity against an unexpected H-2 d haplotype in MRL/lpr (H-2 k) autoimmune disease-prone mice. Immunogenetics 25, 251–257 (1987). https://doi.org/10.1007/BF00404695

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  • DOI: https://doi.org/10.1007/BF00404695

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