Summary
Continuously growing T- and B-cell lines were derived from peripheral blood, affected skin, and lymph nodes of patients with mycosis fungoides (MF) and Sézary syndrome (SS). Two lymphoblastoid cell lines (MF-13 and SS-2) were Epstein-Barr virus (EBV)-transformed B cells evaluated by surface immunoglobulin, lack of E-rosette formation, positive EBV nuclear antibody test, and secretion of IgM antibody in a plaqueforming cell assay. Analysis of the natural-killer-cell activity using peripheral blood lymphocytes from patients with MF and healthy control persons towards MF-13 and SS-2 target cells suggested resistance to lysis even in tests supplemented with 1,000 IU/ml human γ-interferon. However, the cell lines were not per se completely resistant to lysis because lymphocytes from control persons showed significant cytotoxicity in an 18-h assay supplemented with 2 μg/ml concanavalin A.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Edelson RL (1980) Cutaneous T-cell lymphoma: mycosis fungoides, Sézary syndrome, and other variants. J Am Acad Dermatol 2:89–106
Gallo RC, Balttner WA, Reitz MS Jr, Ito Y (1982) HTLV: the virus of adult T cell leukemia in Japan and elsewhere. Lancet I:683
Haynes BF, Bunn P, Mann D, Thomas C, Eisenborth GS, Minna J, Fauci AS (1981) Cell surface differentiation antigens of the malignant T cell in Sézary syndrome and mycosis fungoides. J Clin Invest 67:523–30
Herberman RB, Holden HT (1979) Natural killer cells as antitumor effector cells. J Natl Cancer Inst 62:441–445
Jensen JR, Thestrup-Pedersen K, Ahrons S, Zachariae H (1982) The immunological profile of mycosis fungoides. Clin Exp Immunol 50:397–405
Jensen JR, Sand TT, Jørgensen AS, Thestrup-Pedersen K (1984) Modulation of natural killer cell activity in patients with atopic dermatitis. J Invest Dermatol 82:30–34
Kaltoft K, Thestrup-Pedersen K, Jensen JR, Bisballe S, Zachariae H (1984) Establishment of T- and B-cell lines from patients with mycosis fungoides. Br J Dermatol 111:303–308
Kaltoft K, Bisballe S, Rasmussen K (1985) Balanced terminal chromosome translocations develop in EBV-derived, but non-immortal cell lines from patients with mycosis fungoides. Acta Derm Venereol (Stockh) [Suppl] 120:60–67
Keren Z, Berke G (1984) The porthway of lectin-mediated lymphocytotoxicity by Con A-coupled sepharose beads. Cell Immunol 85:556–562
MacLeunan ICM, Loewi G (1968) Effect of specific antibody to target cells on their specific and non-specific interaction with lymphocytes. Nature 219:1069–1070
Poiesz BJ, Ruscetti FW, Gazdor AF, Bunn PA, Minna JD, Gallo RC (1980) Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T cell lymphoma. Proc Natl Acad Sci USA 77:7415–7419
Poiesz BJ, Ruscetti FW, Reitz MS, Kalyanaraman VS, Gallo RC (1981) Isolation of a new type C retrovirus (HTLV) in primary uncultured cells of a patient with Sézary T-cell leukemia. Nature 294:268–271
Pross HF, Baines MG (1978) Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. Cancer Immunol Immunother 3:75–85
Yamamoto N, Matsumoto T, Koyanagi Y, Tanaka Y, Hinuma Y (1982) Unique cell lines harbouring both Epstein-Barr virus and adult T-cell leukemia virus, established from leukemia patients. Nature 299:367–369
Yamamoto N, Okada M, Koyanagi Y, Kannagi M, Hinuma Y (1982) Transformation of human leukocytes by cocultivation with an adult T cell leukemia virus producer cell line. Science 217:737–739
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jensen, J.R., Kaltoft, K., Bisballe, S. et al. Natural and concanavalin A-induced cytotoxic activity towards continuously growing B lymphocytes derived from patients with cutaneous T-cell lymphoma. Arch Dermatol Res 279, 12–15 (1986). https://doi.org/10.1007/BF00404351
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00404351