Summary
Pemphigus autoantibodies, bound in the epidermis during different stages of acantholysis, were demonstrated with a new techniques for immunoelectron microscopy. Peroxidase-labeled Protein A was used as a new and specific tracer for tissue-bound antibodies of the IgG-type. Advantages were: (1) A small molecular weight of the tracer, (2) a rapid tissue penetration, and (3) shortened incubation times, thus better preserved tissue fine structures. Unspecific adsorption in tissues and on cells was found to be comparatively low.
Zusammenfassung
Pemphigus-Autoantikörper in der Epidermis wurden während verschiedener Stadien der fortschreitenden Acantholyse mit einer neuen immunelektronenmikroskopischen Technik dargestellt. Peroxidase-markiertes Protein A wurde als ein neuer und spezifischer Tracer für gewebegebundene Antikörper vom Typ IgG eingesetzt. Hierbei zeigten sich als Vorteile dieses neuen Nachweisreagenz 1. sein relativ geringes Molekulargewicht, 2. seine Fähigkeit zur raschen Gewebepenetration, und 3. die durch verkürzte Inkubationszeiten verbesserte Erhaltung der feingeweblichen Struktur. Die unspezifische Adsorption im Gewebe und auf Zellen war vergleichsweise gering.
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Trost, T.H., Steigleder, G.K. & Bodeux, E. Immuno-electron microscopical features of in vivo antibody binding during pemphigus blister formation. Arch Dermatol Res 270, 463–468 (1981). https://doi.org/10.1007/BF00403791
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DOI: https://doi.org/10.1007/BF00403791