Abstract
Transient-expression analysis has shown anaerobic regulation of the alcohol dehydrogenase (Adh1) promoter in a chimeric construct. Chimeric plasmids containing the promoter for the Adh1 gene of maize (Zea mays L.) linked to the coding sequence of the chloramphenicol acetyl transferase (cat) gene were introduced into maize protoplasts using electroporation. Both the introduced Adh1 promoter and the endogenous Adh1 gene promoter are regulated at the RNA level, the O2-tension optimum for induction is the same for both, and both promoters initiate transcription from the same site. The demonstration of regulation of a plant gene promoter in a transient expression system will allow the identification of cis acting sequences responsible for regulation.
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Abbreviations
- ADH:
-
alcohol dehydrogenase
- adh :
-
ADH genes
- CAT:
-
chloramphenicol acetyl transferase enzyme
- cat :
-
CAT gene
- Nos :
-
nopaline synthase gene
References
Bevan, M., Barnes, W.M., Chilton, M.-D. (1983) Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Res. 11, 369–385
Chourey, P., Zurawski, D.B. (1981) Callus formation from protoplasts of a maize cell culture. Theor. Appl. Genet. 59, 341–344
Dennis, E.S., Gerlach, W.L., Pryor, A.J., Bennetzen, J.L., Inglis, A., Llewellyn, D., Sachs, M.M., Ferl, R.J., Peacock, W.J. (1984) Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res. 10, 3983–3999
Franck, A., Guilley, H., Janard, G., Richards, K., Hirth, L. (1980) Nucleotide sequence of cauliflower mosaic virus DNA. Cell 21, 285–294
Fromm, M., Taylor, L.P., Walbot, V. (1985) Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc. Natl. Acad. Sci. USA 82, 5822–5828
Gerlach, W.L., Pryor, A.J., Dennis, E.S. Ferl, R.J., Sachs, M.M., Peacock, W.J. (1982) cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize. Proc. Natl. Acad. Sci. USA 79, 2981–2985
Green, C.E., Phillips, R.L. (1975) Plant regeneration from tissue cultures of maize. Crop Sci. 15, 417–421
Gorman, C.M., Moffat, L.F., Howard, B.H. (1982) Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 2, 1044–1051
Hageman, R.H., Flesher, D. (1960) The effect of an anaerobic environment on the activity of alcohol dehydrogenase and other enzymes of corn seedlings. Arch. Biochem. Biophys. 87, 203–209
Hanson, A.D., Jacobsen, J.V. (1984) Control of lactate dehydrogenase, lactate glycolysis, and α-amylase by O2 deficit in barley aleurone layers. Plant Physiol. 75, 566–572
Herrera-Estrella, L., Depicker, A., Van Montagu, M., Schell J. (1983) Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector. Nature 303, 209–213
Howard, E.A., Danna, K.D., Dennis, E.S., Peacock, W.J. (1985) Transient expression in maize protoplasts. In: Plant genetics, pp. 225–234, Freeling, M., ed. Alan R. Liss, New York
Hudson, G.S., Farrell, P.J., Barrell, B.G. (1985) Two related but differentially expressed potential membrane proteins encoded by the EcoRI Dhet region of Epstein-Barr virus B95-8. J. Virol. 53, 528–535
Potrykus, I., Harms, C.T., Lörz, H. (1979) Callus formation from cell culture protoplasts of corn (Zea mays L.). Theor. Appl. Genet. 54, 209–214
Potrykus, I., Shillito, R.D., Saul, M.W., Paszkowski, J. (1985) Direct gene transfer State of the art and future potential. Plant Mol. Biol. Rep. 3, 117–128
Sachs, M., Freeling, M., Okimoto, R. (1980) The anaerobic proteins of maize. Cell 20, 761–767
Taylor, B., Powell, A. (1982) Isolation of plant DNA and RNA. Focus 4, 4–6
Timko, M.P., Kausch, A.P., Castesana, C., Fassler, J., Herrera-Estrella, L., Vanden Broek, G., Van Montagu, M., Schell, J., Cashmore, A.R. (1985) Light regulation of plant gene expression by an upstream enhancer-like element. Nature 318, 579–582
Vayda, M.E., Freeling, M. (1986) Insertion of the M1 transposable element into the first intron of maize Adh1 interferes with transcript elongation but does not disrupt chromatin structure. Plant Mol. Biol. 6, 441–454
Weaver, R.F., Weissman, C. (1979) Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ terminus of 15S β-globin mRNA precursor and mature 10S β-globin mRNA have identical map coordinates. Nucl. Acids Res. 7, 1175–1193
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Howard, E.A., Walker, J.C., Dennis, E.S. et al. Regulated expression of an alcohol dehydrogenase 1 chimeric gene introduced into maize protoplasts. Planta 170, 535–540 (1987). https://doi.org/10.1007/BF00402988
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DOI: https://doi.org/10.1007/BF00402988