Abstract
The thermosensitivity of delayed fluorescence, the relative values of variable chlorophyll fluorescence and the degree of quenching of 9-aminoacridine fluorescene were studied in the chloroplasts from heat-acclimated and non-acclimated (treated 6 h at 52,5°C) young bean plants. The temperature sensitivity of each parameter studied was defined by that temperature at which chloroplast activity decreased by 50% (T50) of its maximum value. There was appreciable increase in the thermostability of membrane energization in chloroplasts isolated from acclimated and non-acclimated plants compared with the controls. The photosynthetic parameters differed according to the suspending medium and the preacclimation treatment. When chloroplast were suspended in phosphate buffer with the addition of stabilizing compounds (2 M sucrose or 0.5% human serum albumin) the thermostability of the thylakoid membranes increased, as was evident by the increases in T50 of about 8–10° C (sucrose) and 2–5° C (human serum albumin) for all the parameters investigated. Photoinduced quenching of 9-aminoacridine fluorescence decreased to some extent in the presence of protective compounds, but in chloroplasts from acclimated plants the T50 was practically equal to that for their long-lived luminescence under the same conditions. At the thylakoid membrane level, acclimation was clearly manifested as an increased thermostability of photoinduced proton-gradient formation.
Similar content being viewed by others
Abbreviations
- 9-AA:
-
9-aminoacridine
- Chl:
-
chlorophyll
- DF:
-
delayed fluorescence
- HSA:
-
human serum albumin
- NB:
-
norman buffer
- PSI (II):
-
photosystem I (II)
- T50:
-
temperature of 50% inhibition of photosynthetic parameter
References
Alexandrov, V.Y. (1977) Cells, molecules and temperature. Comformational flexibility of macromolecules and ecological adaptation. Ecol. Stud. 21, 122–242
Armond, P.A., J.L. Hess (1979) Enhancement of high temperature stability of protein-protein interaction by deuterium oxide. Carnegie Inst. Washington, Yearb. 78, 168–171
Berry, J.A. (1975) Adaptation of photosynthetic processes to stress. Science 188, 644–650
Berry, J., Björkman, O. (1980) Photosynthetic response and adaptation to temperature in higher plants. Annu. Rev. Plant Physiol. 31, 491–543
Emmett, J.M., Walker, D.A. (1968) Thermal uncoupling in chloroplasts. Biochim. Biophys. Acta 180, 424–425
Emmett, J.M., Walker D.A. (1973) Thermal uncoupling in chloroplasts. Inhibition of phosphorylation without depression of light-induced pH change. Arch. Biochem. Biophys. 157, 106–113
Fork, D.C., Murata, N. (1977) Studies on the effect of transition of the physic phase of membrane lipids on electron transport in the extreme thermophyle Synechococcus lividus. Carnegie Inst. Washington, Yearb. 76, 222–226
Gekko, K., Timasheff, S.N. (1981) Mechanism of protein stabilization by glycerol preferential hydration in glycerol-water mixtures. Biochemistry 20, 4667–4676
Goldfeld, M.G., Dmitrovskii, L.G., Blumenfeld, L.A. (1978) Effectiveness of photophosphorylation of chloroplasts in stationary and impulse illumination. Mol. Biol. (USSR) 12, 179–190
Karapetyan, N.V. (1977) Variable fluorescence of chlorophyll in photosynthesis. Usp. Sovrem. Biol. 83, 370–386
Krause, G.H., Santarius, K. (1975) Relative thermostability of the chloroplast envelope. Planta 127, 285–299
Lavorel, J. (1975) Luminescence. In: Bioenergetics of photosynthesis, pp. 223–317, Govindjee, ed. Academic Press, London New York
Malkin, S. (1977) Delayed luminescence. In: Encyclopedia of plant physiology, N.S., vol. 5: Photosynthesis I, pp. 472–491, Trebst, A., Avron, M., eds. Springer, Berlin Heidelberg New York
McCarty, R.E., Jagendorf, A.T. (1965) Chloroplast damage due to enzymatic hydrolysis of endogenous lipids. Plant Physiol. 40, 725–735
Molotkovsky, Y.G., Zhestkova, I.M. (1965) The influence of heating on morphology and photochemical activity of isolated chloroplasts. Biochem. Biophys. Res. Commun. 20, 411–415
Papageorgiou, G. (1975) Chlorophyll fluorescence: An intrinsic probe of photosynthesis. In: Bioenergetics of photosynthesis, pp. 319–371, Govindjee, ed. Academic Press, London New York
Pearcy, R.W. (1978) Effect of growth temperature on the fatty acid composition of the leaf lipids in Atriplex lentiformis (Torr) Wast. Plant Physiol. 61, 484–486
Raison, J.K., Berry, J.A. (1978) The physical properties of membrane lipids in relation to the adaptation of higher plants and algae to contrasting thermal regimes. Carnegie Inst. Washington, Yearb. 77, 276–282
Raison, J.K., Roberts, J.K.M., Berry, J.A. (1982) Correlation between the thermal stability of chloroplast (thylakoid) membranes and the composition and fluidity of their polar lipids upon acclimation of the higher plant Nerium oleander to growth temperature. Biochim. Biophys. Acta 688, 218–228
Santarius, K.A. (1973) The protective effect of sugars on chloroplast membranes during temperature and water stress and its relationship to frost, desiccation and heat resistance. Planta 113, 105–114
Santarius, K.A. (1980) Membrane lipids in heat injury of spinach chloroplasts. Physiol. Plant. 49, 1–6
Santarius, K.A., Müller, M. (1979) Investigation on heat resistance of spinach leaves. Planta 146, 529–538
Schuldiner, S., Rottenberg, H., Avron, M. (1972) Determination of Δ pH in chloroplasts. 2. Fluorescent amines as a probe for determination of Δ pH in chloroplasts. Eur. J. Biochem. 25, 64–70
Venediktov, P.S., Krivoshejeva, A.A. (1983) The mechanisms of fatty-acid inhibition of electron transport in chloroplasts. Planta 159, 411–414
Venediktov, P.S., Krivoshejeva, A.A. (1984) Effect of pH and deuterium oxide on the heat inactivation temperature of chloroplasts. Planta 160, 200–203
Volger, H., Santarius, K.A. (1981) Release of membrane proteins in relation to heat injury of spinach chloroplast. Physiol. Plant. 51, 195–200
Weis, E. (1982) The influence of metal cations and pH on the heat sensitivity of photosynthetic oxygen evolution and chlorophyll fluorescence in spinach chloroplasts. Planta 154, 41–47
Weis, E. (1984) Temperature-induced changes in the distribution of excitation energy between photosystem I and photosystem II in spinach leaves. In: Advances in photosynthesis research, vol. III, pp. 291–294, C. Sybesma, ed. M. Nijhoff/Dr. W. Junk, The Hague Boston Lancaster
Whatley, F.R., Arnon, D.I. (1963) Photosynthetic phosphorylation in plants. Methods Enzymol. 6, 308–313
Wraight, C.A., Crofts, A.R. (1971) Delayed fluorescence and the high-energy state of chloroplasts. Eur. J. Biochem. 19, 386–397
Yamashita, T., Butler, W.L. (1968) Inhibition of chloroplasts by UV-irradiation and heat treatment. Plant Physiol. 43, 2037–2040
Yordanov, I.T. (1981) Photosynthetic chracteristics of Phaseolus vulgaris plants adaptedto high temperatures. In: Photosynthesis. VI. Photosynthesis, and environment, pp. 379–388, Akoyunoglou, G., ed. Balaban Intern. Sci. Services, Philadelphia
Yordanov, I., Goltsev, V., Stoyanova, Ts. (1982) Changes in luminescence characteristics of Phaseolus vulgaris leaves during heat adaptation of young plants. Plant Physiol. (Sofia) 6, 515–518
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yordanov, I., Goltsev, V., Stoyanova, T. et al. High-temperature damage and acclimation of the photosynthetic apparatus. Planta 170, 471–477 (1987). https://doi.org/10.1007/BF00402981
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00402981