Skip to main content
SpringerLink
Log in

Effect of dietary omega-3 and omega-6 fatty acid sources on PUVA-induced cutaneous toxicity and tumorigenesis in the hairless mouse

  • Original Contributions
  • Published:
Archives of Dermatological Research Aims and scope Submit manuscript

Abstract

Because of concern about psoralen-induced phototoxicity and photocarcinogenesis, we investigated the effects of dietary lipids in a mouse model in which 8-methoxypsoralen (8-MOP) and UVA (PUVA) therapy has been shown to be carcinogenic. SKH-Hr-1 hairless albino mice were fed diets containing either omega-3 or omega-6 fatty-acid sources (menhaden oil and corn oil, respectively). After 2 weeks on the diets, the mice were treated topically with 8-MOP and then exposed to UVA (5 J/cm2). Mice receiving the omega-3 fatty-acid source exhibited a marked decrease in inflammatory response and a more rapid repair, as expressed both grossly and microscopically. In support of the latter response, i.e. repair, ornithine decarboxylase activity was about 20% greater in animals receiving the omega-3 fatty-acid source. The effects of the dietary fatty acid sources on PUVA tumorigenesis were examined in long-term studies in which animals were treated topically with 0.01% 8-MOP thrice weekly after which they were exposed to UVA (1 J/cm2). These studies indicated that a dietary lipid rich in omega-3 fatty acid and known to exhibit anti-inflammatory properties can markedly ameliorate the course of PUVA toxicity but does not impede the course of PUVA tumorigenesis

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bang HO, Dyerberg J (1971) Plasma lipid and lipoprotein pattern in Greenlandic West Coast Eskimos. Lancet 1: 1143–1145

    Google Scholar 

  2. Bang HO, Dyerberg J, Hjorne N (1976) The composition of food consumed by Greenland Eskimos. Acta Med Scand 200: 69–73

    Google Scholar 

  3. Black AK, Greaves MW, Hensby CN, Plummer NA (1978) Increased prostaglandins E2 and 335-01 in human skin at 6 and 24 hours after ultraviolet B irradiation (290–320 nm). Br J Clin Pharmacol 5: 431–436

    Google Scholar 

  4. Black HS, Lenger WA, Gerguis J, Thornby JI (1985) Relation of antioxidants and level of dietary lipid to epidermal lipid peroxidation and ultraviolet carcinogenesis. Cancer Res 45: 6254–6259

    Google Scholar 

  5. Black HS, Young AR, Gibbs NK (1989) Effects of butylated hydroxytoluene upon PUVA-tumorigenesis and induction of ornithine decarboxylase activity in the mouse. J Photochem Photobiol 3: 91–100

    Google Scholar 

  6. Camp RD, Greaves MW, Hensby CN, Plummer NA, Warin AP (1978) Irradiation of human skin by short wavelength ultraviolet radiation (100–290 nm) (UVC): increased concentrations of arachidonic acid and prostaglandins E2 and 335-02. Br J Clin Pharmacol 6: 145–148

    Google Scholar 

  7. Conner MJ, Lowe NJ (1984) The induction of erythema, edema, and the polyamine synthesis enzymes ornithine decarboxylase and s-adenosyl-1-methionine decarboxylase in hairless mouse skin by psoralens and longwave ultraviolet light. Photochem Photobiol 39: 787–792

    Google Scholar 

  8. Eaglestein W, Marsico A (1975) Dichotomy in response to indomethacin in UV-C and UV-B induced ultraviolet light inflammation. J Invest Dermatol 65: 238–240

    Google Scholar 

  9. Fischer MA, Black HS (1991) Modification of membrane composition, eicosanoid metabolism, and immunoresponsiveness by dietary omega-3 and omega-6 fatty acid sources, modulators of ultraviolet carcinogenesis. Photochem Photobiol 54: 381–387

    Google Scholar 

  10. Gibbs NK, Honigsmann H, Young AR (1986) PUVA treatment strategies and cancer risk. Lancet 1: 150–151

    Google Scholar 

  11. Henderson CD, Black HS, Wolf JE Jr (1989) Influence of omega-3 and omega-6 fatty acid sources on prostaglandin levels in mice. Lipids 24: 502–505

    Google Scholar 

  12. Horrobin DF (1983) The regulation of prostaglandin biosynthesis by the manipulation of essential fatty acid metabolism. Rev Pure Appl Pharmacol Sci 4: 339–384

    Google Scholar 

  13. Kono T, Mizuno N, Taniguchi S, Ishii M, Yoshida H, Matsui-Yuasaa I, Otani S, Hamada T (1990) PUVA increases ornithine decarboxylase gene expression in mouse skin. Dermatologica 181: 334–337

    Google Scholar 

  14. Kromann N, Green A (1980) Epidemiological studies in the Upernavik District, Greenland. Acta Med Scand 208: 401–406

    Google Scholar 

  15. Lee TH, Hoover RL, Williams JD, Sperling RI, Ravalese J III, Spur BW, Robinson DR, Corey EJ, Lewis RA, Austen KF (1985) Effect of dietary enrichment with eicosapentaenoic and docosahexaenoic acid on in vitro neutrophil and monocyte leukotriene generation and neutrophil function. N Engl J Med 312: 1217–1224

    Google Scholar 

  16. Maurice PDL, Allen BR, Barkely ASJ, Cockbill SR, Stammers J, Barther PC (1987) The effects of dietary supplementation with fish oil in patients with psoriasis. Br J Dermatol 117: 599–606

    Google Scholar 

  17. Miller CC, Ziboh VA (1990) Induction of epidermal hyperproliferation by topical n-3 polyunsaturated fatty acids on guinea pig skin linked to decreased levels of 13-hydroxyoctadecadienoic acid (13-Hode). J Invest Dermatol 94: 353–358

    Google Scholar 

  18. Morison WL, Paul BS, Parrish JA (1977) The effects of indomethacin on long-wave ultraviolet-induced delayed erythema. J Invest Dermatol 68: 120–133

    Google Scholar 

  19. O'Neal MA, Griffin AC (1957) The effect of oxypsoralen upon ultraviolet carcinogenesis in albino mice. Cancer Res 17: 911–916

    Google Scholar 

  20. Orengo IF, Black HS, Kettler AH, Wolf JE Jr (1989) Influence of dietary menhaden oil upon carcinogenesis and various cutaneous responses to ultraviolet radiation. Photochem Photobiol 49: 71–77

    Google Scholar 

  21. Parrish JA, Fitzpatrick TB, Tennenbaum L, Pathak MA (1974) Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light, N Engl. J Med 291: 1207–1211

    Google Scholar 

  22. Plummer NA, Hensby CN, Warin AP, Camp RD, Greaves MW (1978) Prostaglandins E2 and 336-01 and arachidonic acid levels in irradiated and unirradiated skin of psoriatic patients receiving PUVA treatment. Clin Exp Dermatol 3: 367–369

    Google Scholar 

  23. Popp-Snijders C, Schouten JA, Meer J van der, Veen EA van der (1986) Fatty fish-induced changes in membrane lipid composition and viscosity of human erythrocyte suspensions. Scand J Clin Lab Invest 46: 253–258

    Google Scholar 

  24. Punnonen K, Jansen CT, Puntal AA, Ahotaupa M (1991) Effects of in vitro UVA irradiation and PUVA treatment on membrane fatty acids and activities of antioxidant enzymes in human keratinocytes. J Invest Dermatol 96: 255–259

    Google Scholar 

  25. Sheehan DC, Hrapchak BB (1980) Theory and practice of histotechnology. CV Mosby, St Louis

    Google Scholar 

  26. Soyland E, Funk J, Rajka G, Sandberg M, Thune P, Rustad L, Helland S, Middelfart K, Odu S, Falk ES, Solvoll K, Bjornboe G-EA, Drevon CA (1993) Effect of dietary supplementation with very-long-chain n-3 fatty acids in patients with psoriasis. N Engl J Med 328: 1812–1816

    Google Scholar 

  27. Stern RS, Thibodeau LA, Kleinerrman RA, Parrish JA, Fitzpatrick TB (1979) Risk of cutaneous carcinoma in patients treated with oral methoxsalen photochemotherapy for psorasis. N Engl J Med 300: 809–813

    Google Scholar 

  28. Takigawa M, Verma AK, Simsiman RC, Boutwell RK (1982) Polyamine biosynthesis and skin tumor promotion: inhibition of 12-O-tetradecanoylphorbol-13-acetate-promoted mouse skin tumor formation by the irreversible inhibitor of ornithine decarboxylase alpha-difluoromethylornithine. Biochem Biophys Res Commun 105: 969–976

    Google Scholar 

  29. Young AR, Magnus IA, Davies AC, Smith NP (1983) A comparison of the phototumorigenic potential of 8-MOP and 5-MOP in hairless albino mice exposed to solar simulated radiation. Br J Dermatol 108: 507–518

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Dermatology, Baylor College of Medicine, Houston

    A. Yen, H. S. Black & J. Tschen

  2. Veterans Affairs Medical Center, 77030, Houston, TX, USA

    H. S. Black

Authors
  1. A. Yen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. S. Black
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Tschen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yen, A., Black, H.S. & Tschen, J. Effect of dietary omega-3 and omega-6 fatty acid sources on PUVA-induced cutaneous toxicity and tumorigenesis in the hairless mouse. Arch Dermatol Res 286, 331–336 (1994). https://doi.org/10.1007/BF00402224

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00402224

Key words

Search

Navigation