Abstract
A Rhizobium trifolii symbiotic plasmid specific gene library was constructed and the physical organisation of regions homologous to nifHDK, nifA and nod genes was determined. These symbiotic gene regions were localised to u 25 kb region on the sym-plasmid, pPN1. In addition four copies of a reiterated sequence were identified on this plasmid, with one copy adjacent to nifH. No rearrangement of these reiterated sequences was observed between R. trifolii bacterial and bacteroid DNA. Analysis of a deletion derivative of pPN1 showed that these sequences were spread over a 110 kb region to the left of nifA.
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Scott, D.B., Court, C.B., Ronson, C.W. et al. Organisation of nodulation and nitrogen fixation genes on a Rhizobium trifolii symbiotic plasmid. Arch. Microbiol. 139, 151–157 (1984). https://doi.org/10.1007/BF00401991
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DOI: https://doi.org/10.1007/BF00401991