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High resolution studies on the pattern of induced exchanges in the human karyotype

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Abstract

The pattern of breakage and exchange induced by X-irradiation of human lymphocytes at G1 has been analysed in PHA transformed cultures at X1 metaphase in cells treated with trypsin. All the events observed occurred in interband segments. Moreover, as far as the autosomes were concerned, these events appear to be at random in relation to trypsin interband length but give some indication of non-randomness when total chromosome length is considered. The X and Y chromosomes, on the other hand, show far fewer breaks than would be expected whichever criterion is adopted, and in particular appear to be isolated from the autosomes with respect to the occurrence of exchange events. — The analysis of specific break points in relation to trypsin banding sequences, makes it clear that conclusions regarding chromosome rearrangements based solely on conventional preparations may be misleading.

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  1. Department of Zoology, University of Southampton, England

    Marina Seabright

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  1. Marina Seabright
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Seabright, M. High resolution studies on the pattern of induced exchanges in the human karyotype. Chromosoma 40, 333–346 (1973). https://doi.org/10.1007/BF00399426

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  • DOI: https://doi.org/10.1007/BF00399426

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