Abstract
Using the metabolic-inhibition (MI) test as an assay, sera from rabbits immunized with Mycoplasma pneumoniae were separated on the basis of a requirement for a heat-labile (56 C) accessory factor (HLAF). As in previous studies, this requirement was found in sera collected early in the immune response, but disappeared as the immunization procedure progressed. Results obtained following exposure of HLAF-requiring sera to zymosan, hydrazine, and ethylene-diaminetetraacetic acid (EDTA) suggested that complement components C3, C8, and possible other components which follow C3 in the complement cascade, were required to demonstrate MI activity. Results obtained when HLAF-non-requiring sera were treated in the same manner, suggested the presence of two antibody populations. One population required component C3, and possibly C5, C6, and C9, while the other population required none of the complement components.
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Barrett, J. T. 1970. The immunochemistry of complement, p. 139–153. In J. T. Barrett, Textbook of Immunology. —C. V. Mosby Co., St. Louis, Mo., U.S.A.
Coleman, L. H. and Lynn, R. J. 1972a. Immune inhibition of Mycoplasma pneumoniae. —Proc. Soc. Exp. Biol. Med. 140: 383–386.
Coleman, L. H. and Lynn, R. J. 1972b. Heat lability of rabbit-anti-Mycoplasma pneumoniae serum.— Antonie van Leeuwenhoek 38: 193–200.
Cushing, J. E.Jr. 1945. A comparative study of complement.— J. Immunol. 50: 75–89.
Fernald, G. W., Clyde, W. A.Jr. and Denny, F. W. 1967a. Factors influencing growth inhibition of Mycoplasma pneumoniae by immune sera.— Proc. Soc. Exp. Biol. Med. 125: 161–166.
Fernald, G. W., Clyde, W. A.Jr. and Denny, F. W. 1967b. Nature of the immune response to Mycoplasma pneumoniae.— J. Immunol. 98: 1028–1038.
Gale, J. L., and Kenny, G. E. 1970. Complement dependent killing of Mycoplasma pneumoniae by antibody: Kinetics of the reaction.— J. Immunol. 104: 1175–1183.
Kempf, R. A., Gigli, I. and Austen, K. F. 1969. Inhibition of the lytic effect of guinea pig complement by rabbit complement.— J. Immunol. 102: 795–803.
Klein, P. G. and Wellensiek, H. J. 1965. Multiple nature of the third component of guinea pig complement. I. Separation and characterization of three factors, a, b, and c, essential for hemolysis.— Immunology 8: 590–603.
Lepow, I. H., Naff, G. B., Todd, E. W., Pensky, J., and Hinz, C. F.Jr. 1963. Chromatographic resolution of the first component of human complement into three activities.—J. Exp. Med. 117: 983–1008.
Mardiney, M. R.Jr. and Müller-Eberhard, H. J. 1965. Mouse B IC Globulin: Production of antiserum and characterization in the complement reaction.— J. Immunol. 94: 877–882.
Muir, R. 1912. Relationships between the complements and immune bodies of different animals; the mode of action of immune body.— J. Pathol. Bacteriol. 16: 523–534.
Müller-Eberhard, H. J. 1968. Chemistry and reaction mechanisms of complement.—Advan. Immunol. 8: 1–80.
Riggs, S., Sharp, J. T. and Carpenter, R. R. 1967. Factors involved in growth-inhibition of mycoplasma by immune serum.— Ann. N. Y. Acad. Sci. 143: 784–793.
Turk, J. L. 1959. The relationship between complement and antibodies of different animals in the immune adherance phenomenon.— Immunology 2: 127–136.
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Coleman, L.H., Lynn, R.J. & Patrick, R.A. Nature of the serum heat-labile accessory factor involved in the metabolic inhibition of Mycoplasma pneumoniae . Antonie van Leeuwenhoek 40, 401–407 (1974). https://doi.org/10.1007/BF00399352
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DOI: https://doi.org/10.1007/BF00399352