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Characterization of 14C-naphthol uptake in excised root segments of clover (Trifolium pratense L.) and fescue (Festuca arundinaceae Screb.)

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Abstract

An isolated root uptake test (IRUT) was used to characterize the bioaccumulation of 14C-naphthol in excised root segments obtained from 6-month-old hydroponically grown plants: two varieties of fescue (Festuca arundinaceae Schreb.) and one variety of clover (Trifolium pratense L.). Naphthol uptake rates were directly related to naphthol concentration in the range 0.01 to 0.2 uM. The incubation time required for equilibrium to be reached between naphthol in root tissue and in solution was between 9 and 12 h. Tests using metabolic inhibitors, KCN, NaN3, and DNP, indicated that naphthol uptake may be the result of both passive and active mechanisms. Q10 values for naphthol uptake ranged from 1.05 to 1.16.

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Authors and Affiliations

  1. Waste Management Institute, University of Tennessee, Knoxville, TN, USA

    Michael A. Kristich & Otto J. Schwarz

  2. Botany Department, University of Tennessee, Knoxville, TN, USA

    Michael A. Kristich & Otto J. Schwarz

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  1. Michael A. Kristich
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  2. Otto J. Schwarz
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Kristich, M.A., Schwarz, O.J. Characterization of 14C-naphthol uptake in excised root segments of clover (Trifolium pratense L.) and fescue (Festuca arundinaceae Screb.). Environ Monit Assess 13, 35–44 (1989). https://doi.org/10.1007/BF00398734

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