Summary
Using the aphid stylet technique 14C ATP was shown to be readily taken up into the sieve elements of willow. At the same time this compound was found to be metabolised during uptake resulting in labelled ADP and AMP appearing in the stylet exudate. Longitudinal movement of labelled ATP was also found to occur.
Measurement of the levels of ATP and ADP in stylet exudate showed that both were present in high concentrations. The ratio ATP/ADP varied between 2.0 and 5.3.
The effect of certain inhibitors of oxidative phosphorylation (oligomycin and DNP) and glycolysis (fluoride) on the rate of stylet exudation was studied. All three inhibitors caused a cessation of exudation but this did not occur until several hours after inhibitor application. Oligomycin and DNP had no effect on the concentration of ATP in the sap. Fluoride however, appeared in some cases to reduce the ATP concentration to a low level an hour or more before exudation finally stopped.
Incorporation of 32Pi into organic phosphate esters present in stylet exudate was found to occur within 15 minutes of the application of the tracer to a bark strip. Labelling of organic phosphates also took place, at a slower rate, when 32P inorganic phosphate was incubated with stylet exudate.
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References
Aikman, D. P., Anderson, W. P.: A quantitative investigation of a peristaltic model for phloem translocation. Ann. Bot. 35, 761–777 (1971).
Becker, D., Kluge, M., Ziegler, H.: Der Einbau von 32PO4—in organische Verbindungen durch Siebröhrensaft. Planta (Berl.) 99, 154–162 (1971).
Duloy, M., Mercer, F. V., Rathgeber, N.: Studies in translocation. II. Submicroscopic anatomy of the phloem. Aust. J. biol. Sci. 14, 506–518 (1961).
Gardner, D. C. J., Peel, A. J.: ATP in sieve tube sap from willow. Nature (Lond.) 222, 774 (1969).
Kennecke, M., Ziegler, H., Fekete, M. A. R.: Enzymaktivitäten im Siebröhrensaft von Robinia pseudoacacia L. und anderer Baumarten. Planta (Berl.) 98, 330–356 (1971).
Kluge, M., Becker, D., Ziegler, H.: Untersuchungen über ATP und andere organische Phosphorverbindungen im Siebröhrensaft von Yucca flaccida und Salix triandra. Planta (Berl.) 91, 68–79 (1970).
Kluge, M., Ziegler, H.: Der ATP-Gehalt der Siebröhrensäfte von Laubbäumen. Planta (Berl.) 61, 167–177 (1964).
MacRobbie, E. A. C.: Phloem translocation. Facts and mechanism. A comparative survey. Biol. Rev. 46, 429–481 (1971).
Pradet, A.: Etudes des adenosine-5-mono, di, et tri-phosphates dans les tissus vegetaux. I. Dosage enzymatique. Physiol. Veg. 5, 209–221 (1961).
Randerath, K.: Thin layer chromatography. New York: Acad. Press 1966.
Spanner, D. C.: The translocation of sugar in sieve tubes. J. exp. Bot. 9, 332–342 (1958).
Thaine, R.: A translocation hypothesis based on the structure of plant cytoplasm. J. exp. Bot. 13, 152–160 (1962).
Weatherley, P. E., Peel, A. J., Hill, G. P.: The physiology of sieve tube sap. J. exp. Bot. 10, 1–16 (1959).
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Gardner, D.C.J., Peel, A.J. Some observations on the role of ATP in sieve tube translocation. Planta 107, 217–226 (1972). https://doi.org/10.1007/BF00397937
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DOI: https://doi.org/10.1007/BF00397937