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Identification of secretory vesicles in homogenates of pea stem segments

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Abstract

In homogenates of stem sections from etiolated pea (Pisum sativum L.) seedlings, secretory vesicles can be separated from Golgi-apparatus cisternae by rate-zonal centrifugation in renografin gradients. Optically, two bands of turbidity are observed, the uppermost containing the secretory vesicles and the lower one the Golgi-apparatus cisternae. The absence of glutaraldehyde in the homogenizing medium has allowed the effective characterization of marker-enzyme activities. Golgi-apparatus cisternae have been recognized by the presence of inosine-diphosphatase and glucan-synthase I activities as well as by electron microscopy. In contrast, although secretory vesicles also bear inosine diphosphatase they do not appear to possess glucan-synthase activity. Three plasma-membrane markers, NPA-binding, glucan synthase II, and KCl,Mg2+-adenosine triphosphatase (pH 6.5), were not detected in secretory vesicles. Pulse-chase experiments with [3H]glucose support our designation of secretory vesicles and Golgi-cisternal fractions.

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Abbreviations

ER:

endoplasmic reticulum

GSI, GSII:

glucan, synthase I, II, respectively

IDPase:

inosine diphosphatase

PM:

plasma membrane

SV(s):

secretory vesicle(s)

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Authors and Affiliations

  1. Thimann Laboratories, Division of Natural Sciences, University of California, 95064, Santa Cruz, CA, USA

    L. Taiz

  2. Harvard Forest, 01366, Petersham, MA, USA

    M. Murry

  3. Abteilung Cytologie, Pflanzenphysiologisches Institut der Universität, Untere Karspüle 2, D-3400, Göttingen, Germany

    D. G. Robinson

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  1. L. Taiz
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  2. M. Murry
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  3. D. G. Robinson
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Taiz, L., Murry, M. & Robinson, D.G. Identification of secretory vesicles in homogenates of pea stem segments. Planta 158, 534–539 (1983). https://doi.org/10.1007/BF00397244

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  • DOI: https://doi.org/10.1007/BF00397244

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