Summary
The replication of chromosomal DNA in a series of abnormal human cell cultures has been studied by means of DNA-fiber autoradiography. In lymphocytes with trisomy 21, in fibroblasts of 45,X; 47,XXX; 49,XXXXY; and 49,XXXXX chromosomal constitution, and in fibroblasts from a patient with xeroderma pigmentosum (De Sanctis-Cacchione syndrome), the rate of DNA replication does not differ from that in normal cells, varying in a single fork from 0.2 to 1.0 μm/min with a mean of about 0.6 μm/min. In fibroblasts with trisomy 7 the rate of DNA replication is greater, varying from 0.3 to 1.2 μm/min with a mean of about 0.8 μm/min. The sizes of replication units in all cells examined are from 80 to 500 μm with a mean of about 200–300 μm.
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Yurov, Y.B. Replication of chromosomal DNA in cultured abnormal human cells. Hum Genet 43, 47–52 (1978). https://doi.org/10.1007/BF00396477
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DOI: https://doi.org/10.1007/BF00396477