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Assignment of the Ly-6--Ril-1--Sis--H-30--Pol-5/Xmmv-72--Ins-3--Krt-1--Int-1--Gdc-1 region to mouse chromosome 15

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Abstract

Previous work has demonstrated linkage between Ly-6, H-30, and a locus, Ril-1, that affects susceptibility to radiation-induced leukemia. Results of preliminary linkage analyses suggested further that the cluster might be linked to Ly-11 on the proximal portion of mouse chromosome 2. Using molecular probes to examine somatic cell lines and recombinant inbred and congenic strains of mice, we have re-evaluated these linkage relationships. A cloned genomic DNA fragment derived from a retroviral site has been used to define a novel locus, Pol-5, that is tightly linked to both H-30 and Ril-1 as shown by analysis of the B6.C-H-30 c congenic mouse strain. Following the segregation of the Pol-5 mouse-specific DNA fragment in a series of somatic cell hybrids carrying various combinations of mouse chromosomes on a rat or Chinese hamster background mapped Pol-5 to mouse chromosome 15. During the course of these studies, restriction fragment length polymorphisms were defined associated with several loci, including Pol-5, Ly-6, Sis, Ins-3, Krt-1, Int-1, and Gdc-1. Three of these loci, Sis, Int-1, and Gdc-1, have been previously mapped to chromosome 15 by others using somatic cell hybrids or isoenzyme analyses. Following the inheritance of these eight loci in recombinant inbred strains of mice allowed the definition of a linkage group on the chromosome with the order Ly-6-Ril-1--Sis--H-30--Pol-5--Ins-3--Krt-1--Int-1--Gdc-1. Analyses of alleles inherited as passengers in B6.C-H-30 c, C3H.B-Ly-6 b, and C57BL/6By-Eh/+ congenic mouse strains and in situ hybridization experiments support the above gene order and indicate further that the cluster is located on distal chromosome 15, with Ly-6 and Sis near Eh.

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Abbreviations

A :

agouti

Abl :

cellular homolog of the Abelson leukemia virus oncogene

Ada :

adenosine deaminase

Ak-1 :

adenylate kinase-1

AXB:

A/J × C57BL/6J recombinant inbred strain

B2m:

beta-2 microglobulin

BXA:

C57BL/6J × A/J recombinant inbred strain

BXD:

C57BL/6J × DBA/2J recombinant inbred strain

BXH:

C57BL/6J × C3H/HeJ recombinant inbred strain

CXB:

BALB/cBy × C57BL/6By recombinant inbred strain

DNA:

deoxyribonucleic acid

Eh :

hairy ears

Fpgs :

folypolyglutamyl synthetase

FXI:

fractionated x-irradiation

Gdc-1 :

glycerol phosphate dehydrogenase-1

Il2r :

IL-2 receptor

Ins-3 :

a novel insulinlike gene

Int-1 :

mammary tumor integration site-1

Itp :

inosine triphosphatase

Krt-1 :

the locus designated here includes a cluster of at least three keratin genes

LTR:

long terminal repeat

Ly:

lymphocyte

Lv-6:

lymphocyte antigen-6

Ly-11:

lymphocyte antigen-11

MIH:

minor histocompatibility

Myc :

cellular homolog of the Abelson leukemia virus oncogene; pa, pallid;

Pol-5 :

locus encoding retroviral polymerase-5

RFLP:

restriction fragment length polymorphism

RI:

recombinant inbred mouse strains

Ril-1 :

radiation-induced leukemia susceptibility-1 locus

SDP:

strain distribution pattern

Sis :

cellular homolog of the simian sarcoma virus oncogene

SFFV:

spleen focus-forming virus

Tpi-1 :

triosephosphate isomerase-1

Ve :

velvet

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Authors and Affiliations

  1. Pathology and Biochemistry and Kaplan Cancer Center, New York University Medical Center, 550 First Avenue, 10016, New York, NY, USA

    Daniel Meruelo, Anthony Rossomando, Suzanne Scandalise & Peter D'Eustachio

  2. Department of Microbiology and the Comprehensive Cancer Center, University of Southern California School of Medicine, 90033, Los Angeles, CA, USA

    R. E. K. Fournier

  3. Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, 20892, Bethesda, MD, USA

    Dennis R. Roop

  4. Department of Genetics, The Weizmann Institute of Science, 76 100, Rehovot, Israel

    Debra Saxe & Cila Blatt

  5. Department of Biology, University of California at San Diego, 92093, La Jolla, CA, USA

    Muriel N. Nesbitt

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  1. Daniel Meruelo
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  2. Anthony Rossomando
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Meruelo, D., Rossomando, A., Scandalise, S. et al. Assignment of the Ly-6--Ril-1--Sis--H-30--Pol-5/Xmmv-72--Ins-3--Krt-1--Int-1--Gdc-1 region to mouse chromosome 15. Immunogenetics 25, 361–372 (1987). https://doi.org/10.1007/BF00396102

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  • DOI: https://doi.org/10.1007/BF00396102

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