Skip to main content
SpringerLink
Log in

Ethylene metabolism in Pisum sativum L.

  • Published:
Planta Aims and scope Submit manuscript

Abstract

The possible role of C2H4 metabolism in mediating the responses of plants to C2H4 is re-examined. It is demonstrated that (i) the effects of inhibitors upon C2H4 action do not correspond with their effects on metabolism, (ii) elicitors of C2H4 effects do not have appropriate effects on C2H4 metabolism, (iii) inhibitors of C2H4 metabolism do not affect the response of plants to C2H4. It is concluded that metabolism of C2H4 is not linked to the mode of action of the growth regulator.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DTC:

sodium diethyldithiocarbamate

FW:

fresh weight

References

  • Abeles, F.B. (1973) Ethylene in plant biology. Academic Press, New York

    Google Scholar 

  • Abeles, F.B. (1984a) A comparative study of ethylene oxidation in Vicia faba and Mycobacterium paraffinicum. J. Plant Growth Regul. 3, 85–95

    Google Scholar 

  • Abeles, F.B. (1984b) Role of ethylene oxidation in the mechanism of C2H4 action. In: Ethylene: Biochemical, physiological and applied aspects, pp. 75–86, Fuchs, Y., Chalutz, E., eds. Martinus Nijhoff/Dr.W. Junk Publishers, The Hague

    Google Scholar 

  • Abeles, R.B., Ruth, J.M., Forrence, L.E., Leather, G.K. (1972) Mechanism of ethylene action: use of deuterated C2H4 to measure isotopic exchange with plant material and the biological effects of deuterated ethylene. Plant Physiol. 49, 669–675

    Google Scholar 

  • Beyer, E.M. (1972) Mechanism of C2H4 action. Biological activity of deuterated ethylene and evidence against isotopic exchange and cis-trans-isomerization. Plant Physiol. 49, 672–675

    Google Scholar 

  • Beyer, E.M. (1975) 14C2H4: Its incorporation and metabolism by pea seedlings under aseptic conditions. Plant Physiol. 56, 273–278

    Google Scholar 

  • Beyer, E.M. (1976) A potent inhibitor of ethylene action in plants. Plant Physiol. 58, 268–271

    Google Scholar 

  • Beyer, E.M. (1977) 14C2H4: Its incorporation and oxidation to 14CO2 by cut carnations. Plant Physiol. 60, 203–206

    Google Scholar 

  • Beyer, E.M. (1978) Rapid metabolism of propylene by pea seedlings. Plant Physiol. 61, 893–895

    Google Scholar 

  • Beyer, E.M. (1979a) Effect of silver ion, carbon dioxide and oxygen on ethylene action and metabolism. Plant Physiol. 63, 169–173

    Google Scholar 

  • Beyer, E.M. (1979b) [14C] ethylene metabolism during leaf abscission in cotton. Plant Physiol. 64, 971–974

    Google Scholar 

  • Beyer, E.M. (1980) Recent advances in ethylene metabolism. In: Aspects and prospects of plant growth (Dutch Plant Growth Regulator Group/British Plant Growth Regulator Group, Monogr. 6), pp. 35–69, Teffcoat B, ed. Wessex Press, Wantage, UK

    Google Scholar 

  • Beyer, E.M. (1985) Ethylene metabolism. In: Ethylene in plant development, pp. 125–127, Roberts JA, Tucker GA, eds. Butteworths, London

    Google Scholar 

  • Beyer, E.M., Blomstrom, D.C. (1979) Ethylene metabolism and its possible physiological role in plants. In: Plant growth substances, 1979, pp. 208–218, Skoog, F., ed. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Beyer, E.M., Sundin, O. (1978) 14C2H4 Metabolism in morning glory flowers. Plant Physiol. 61, 896–899

    Google Scholar 

  • Burg, S.P., Burg, E.A. (1967) Molecular requirements for the biological activity of ethylene. Plant Physiol. 42, 144–152

    Google Scholar 

  • Cheng, Y., Prusoff, W.H. (1973) Relationship between the inhibition constant (Ki) and the concentration of inhibitior which causes a 50 per cent inhibition (I50) of an enzymic reaction. Biochem. Pharmacol. 22, 3099–3108

    Google Scholar 

  • Colby, J., Stirling, D.I., Dalton, H. (1977) The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath): Its ability to oxygenate n-alkenes, ethers and alicyclic, aromatic and heterocyclic compounds. Biochem. J. 165, 395–402

    Google Scholar 

  • Collman, J.P., Brauman, J.I., Mennier, B., Raybuck, S.A., Kodakek, T. (1984) Epoxidation of olefins by cytochrome P-40 model compounds: Mechanism of oxygen atom transfer. Proc. Natl. Acad. Sci. USA 81, 3245–3248

    Google Scholar 

  • Collman, J.P., Brauman, J.I., Mennier, B., Hayashi, T., Kodadek, T., Raybuck, S.A. (1985) Epoxidation of olefins by cytochrome P-450 model compounds: Kinetics and stereochemistry of oxygen atom transfer and origin of shape selectivity. J. Am. Chem. Soc. 107, 2000–2005

    Google Scholar 

  • Croteau, R., Kolattukudy, P.E. (1975) Enzyme epoxidation of 18-hydroxyoleic acid to 18-hydroxy-cis-9, 10-epoxystearic acid by a particulate preparation from spinach (Spinacia oleracea). Arch. Biochem. Biophys. 170, 612–672

    Google Scholar 

  • Duggan, J., Gassman, M. (1974) Induction of porphyrin synthesis in etiolated bean leaves by chelators of iron. Plant Physiol. 53, 206–215

    Google Scholar 

  • Evans, D.E., Smith, A.R., Taylor, J.E., Hall, M.A. (1984) Ethylene metabolism in Pisum sativum L.: kinetic parameters, the effects of propylene, silver and carbon dioxide and comparison with other systems. Plant Growth Regul. 2, 187–195

    Google Scholar 

  • Filser, J.G., Bolt, H.M. (1983) Exhalation of ethylene oxide by rats on exposure to C2H4. Mutation Res. 120, 57–60

    Google Scholar 

  • Fishbean, L. (1976) Environmental health aspects of fungicides. 1. Dithiocarbamates. J. Toxicol. Environ. Health 1, 713–735

    Google Scholar 

  • Hall, M.A., Mutumba, G.C., Sanders, I.O., Smith, A.R., Starling, R.J., Williams, R.A.N. (1987) Ethylene in seed physiology. In: Growth regulators and seeds (British Plant Growth Regulator Group Monogr. 15, 1987), pp. 53–63, Pinfield, N.J., Black, M., eds. Wessex Press, Wantage, UK

    Google Scholar 

  • Liebermann, M. (1979) Biosynthesis and action of ethylene. Annu. Rev. Plant Physiol. 30, 533–591

    Google Scholar 

  • May, S.W., Lee, L.G., Katopodis, A.G., Kuo, J.-Y., Wimalasena, K. (1984) Rubredoxin from Pseudomonas oleovorans: Effects of selective chemical modification and metal substitution. Biochem. 23, 2187–2192

    Google Scholar 

  • Maynert, E.W., Foreman, R.L., Watabe, T. (1970) Expoxides as obligatory intermediates in the metabolism of olefins to glycols. J. Biol. Chem. 245, 5234–5238

    Google Scholar 

  • McKee, R.W. (1941) Solubility of carbon disulphide vapour in body fluids and tissues. J. Ind. Hyg. Toxicol. 23, 484–489

    Google Scholar 

  • Mellor, D.P., Malley, L. (1947) Stability constants of internal complexes. Nature 159, 370

    Google Scholar 

  • Obrebska, M.J., Kentish, P., Parke, D.V. (1979) The effects of carbon disulphide on rat liver microsomal mixed-function oxidases in vivo and in vitro. Biochem. J. 188, 107–112

    Google Scholar 

  • Ortiz de Montellano, P.R., Mico, B.A. (1980) Destruction of cytochrome P-450 by ethylene and other olefins. Mol. Pharmacol. 18, 128–135

    Google Scholar 

  • Ortiz de Montellano, P.R., Beilan, H.S., Kunze, K.L., Mico, B.A. (1981) Destruction of cytochrome P-450 by ethylene. Structure of the resulting prosthetic heme adduct. J. Biol. Chem. 256, 4395–4399

    Google Scholar 

  • O'Sullivan, W.J. (1969) Stability constants of metal complexes. In: Data for biochemical research, pp. 423–434, Dawson, R.M.C., Elliot, D.C., Elliot, W.H., Jones, K.M., eds. 2nd edn., Oxford University Press

  • Ruettinger, R.T., Fulco, A.J. (1981) Epoxidation of unsaturated fatty acids by a soluble cytochrome-P450-dependent system from Bacillus megatherium. J. Biol. Chem. 256, 5218–5734

    Google Scholar 

  • Sanders, I.O. (1986) Ethylene metabolism in Pisum sativum L. cv. ‘Alaska’. Ph.D. thesis, University College of Wales, Aberystwyth

    Google Scholar 

  • Sanders, I.O., Smith, A.R., Hall, M.A. (1989) The measurement of ethylene binding and metabolism in plant tissue. Planta 179, 78–84

    Google Scholar 

  • Siefermann, D., Yamamoto, H.Y. (1975) Properties of NADPH and oxygen-dependent zeaxanthin epoxidation in isolated chloroplasts. Arch. Biochem. Biophys. 171, 70–77

    Google Scholar 

  • Sisler, E.C., Yang, S.F. (1984) Anti-ethylene effects of cis-2-butene and cyclo olefins. Phytochem. 23, 2765–2768

    Google Scholar 

  • Smith, P.G. (1983) Studies on the site of action of ethylene. Ph.D. thesis, University College of Wales, Aberystwyth

    Google Scholar 

  • Smith, P.G., Venis, M.A., Hall, M.A. (1985) Oxidation of ethylene by cotyledon extracts from Vicia faba L. Planta 163, 97–104

    Google Scholar 

  • Taylor, G.E. (1983) The significance of the developing energy technologies of coal conversion to plant productivity. Hort. Sci. 18, 684–689

    Google Scholar 

  • Venis, M.A. (1985) Hormone binding sites in plants. Longman, New York

    Google Scholar 

  • West, C.A. (1980) Hydroxylases, monooxygenases and cytochrome P-450 In: Biochemistry of plants, vol. 2, pp. 317–364, Davies, D.D., ed. Academic Press, New York London

    Google Scholar 

  • Wilhelm, E., Baltino, R., Wilcock, R.J. (1977) Low pressure solubility of gases in liquid water. Chem. Rev. 77, 219–262

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, University College of Wales, SY23 3DA, Aberystwyth, Dyfed, UK

    I. O. Sanders, A. R. Smith & M. A. Hall

Authors
  1. I. O. Sanders
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. R. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. A. Hall
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sanders, I.O., Smith, A.R. & Hall, M.A. Ethylene metabolism in Pisum sativum L.. Planta 179, 104–114 (1989). https://doi.org/10.1007/BF00395777

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00395777

Key words

Search

Navigation