Abstract
A winter variety (Miranovskaja 808) of wheat (Triticum aestivum L.) was grown in water cultures containing 0, 5, 15, 30, and 60 mM choline chloride. There was an increase in the phosphatidylcholine level at the expense of phosphatidic acid, parallel with an increasing choline concentration in the cultivating medium. While the ratio of free sterols to phospholipids remained essentially constant in the seedlings, there was an increase in the ratio of saturated-to-unsaturated fatty acids of the phospholipid fraction. Probing the protoplasts obtained from the leaves of the seedlings, with spin labeled 5-doxyl- and 16-doxyl stearic acids, indicated a progressive rigidifying of the hydrophobic core of the plasmalemma. It is suggested that this is a manifestation of compensatory mechanisms by which plants attempt to maintain unchanged average membrane fluidity across their membranes in response to the fluidizing effect of choline head groups.
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Folch, J., Less, M., Sloane-Stanley, G.H. (1975) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509
Griffith, O.H., Jost, P.C. (1976) Theory and applications in Spin labeling, pp. 453–523. Berliner, L.J. ed. Academic Press, New York
Horváth, I., Vigh, L., Belea, A., Farkas, T. (1979) Conversion of phosphatidylcholine to phosphatidic acid in freeze injured rye and wheat cultivars. Physiol. Plant. 45, 57–62
Horváth, I., Vigh, L., Farkas, T. (1981) The manipulation of polar head group composition of phospholipids in the wheat Miranovskaja 808 affects frost tolerance. Planta 151, 103–108
Hubbel, W.L., McConnel, H.M. (1971) Molecular motion in spinlabeled phospholipids and membranes. J. Am. Chem. Soc. 93, 314–326
Jacobson, K., Papahadjopoulos, D. (1975) Phase transitions and phase separations in phospholipid membranes induced by changes in temperature, pH, and concentration of bivalent cations. Biochemistry 14, 152–161
McKersie, B.D., Thompson, J.E. (1979) Influence of plant sterols on the phase properties of phospholipid bilayers. Plant Physiol. 63, 802–805
Nandini-Kishore, S.G., Kitajima, S.G., Thompson, G.A. (1977) Membrane fluidizing effects of general anesthetics methoxyflurane elicit and acclimation response in Tetrahymena. Biochim. Biophys. Acta 471, 157–161
Op Den Kamp, J.A., Kauerz, M.T., Van Deenen, L.L.M. (1975) Action of pancreatic phospholipase A2 on phosphatidylcholine bilayers in different physical states. Biochim. Biophys. Acta 406, 169–177
Robert, J., Rebel, G., Mandel, P., Yavin, E. (1978) Polar head groups manipulation of phospholipids in cultured neuroblastoma cells. Life Sci. 22, 211–216
Rouser, G., Fleischer, S., Yamamoto, A. (1970) Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 5, 494–496
Schroeder, F., Perlmutter, J.F., Glasdr, M., Vagelos, P.R. (1976) Isolation and characterisation of subcellular membranes with altered phospholipid composition from cultures fibroblasts. J. Biol. Chem. 251, 5016–5926
Schroeder, F. (1978) Isothermal regulation of membrane fluidity in murine fibroblasts with altered phospholipid polar head groups. Biochim. Biophys. Acta 511, 356–376
Vigh, L., Horváth, I., Farkas, T., Horváth, L.I., Dudits, D. (1979) Protoplasts as a useful tool for studying plant plasma membrane microviscosity. In: Advances in Protoplast Reserch, pp. 457–462. Farkas, G. ed. Academic Press. Publishing House of the Hungarian Academy of Sciences
Varring, A.J., Breidenbach, R.W., Lyons, J.M. (1976) In vivo modification of plant membrane phospholipid composition. Biochim. Biophys. Acta 471, 157–161
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Horváth, I., Vigh, L., Farkas, T. et al. Effect of choline chloride on fatty acid chain ordering in membranes of wheat (Triticum aestvium L. cv. Miranovskaja 808). Planta 153, 476–480 (1981). https://doi.org/10.1007/BF00394990
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DOI: https://doi.org/10.1007/BF00394990