Abstract
Citrobacter freundii Hfr donor strains were isolated from a C. freundii strain harbouring a temperature-sensitive factor F ts 114 lac +, by selecting for integrative suppression of the ts 114 mutation. Three Hfr strains were characterized, which transfer their chromosomes in a linear and oriented order. The first strain transfers: O-aro +-ilv +-pur +-thr +-leu +-pro +, the second: O-ilv +-pur +-thr +-leu +-pro + and the third: O-ilv +-aro +-nad +-his +-pro +. The whole chromosome is transferred into the recipient cell within about 145 minutes. From these results we concluded that the linkage map of C. freundii is circular.
Mating-pair formation on a membrane filter resulted in more recombinants being formed as compared with mating-pair formation in liquid medium. Furthermore the mating-pairs formed on a membrane were more stable. From one Hfr strain heterogenic F-prime factors could be isolated bearing the F ts 114 lac + genes from Escherichia coli and the pur + and/or ilv + genes from C. freundii.
Preliminary mapping by interrupted mating indicated that the linkage map of C. freundii is in general very similar to those of E. coli, Salmonella typhimurium and Klebsiella aerogenes.
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References
Adelberg, E. A., Mandel, M. and Chen, G. C. C. 1965. Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K 12.—Biochem. Biophys. Res. Commun. 18: 788–795.
Beckwith, J. R., Signer, E. R. and Epstein, W. 1966. Transposition of the lac region of E. coli.—Cold Spring Harbor Symp. Quant. Biol. 31: 393–401.
Casse, F., Pascal, M. C. and Chippaux, M. 1973. Comparison between the chromosomal maps of Escherichia coli and Salmonella typhimurium. Length of the inverted segment in the trp region.—Mol. Gen. Genet. 124: 253–257.
Curtiss, R. III, Charamella, L. J., Stallions, D. R. and Mays, J. A. 1968. Parental functions during conjugation in Escherichia coli K-12.—Bacteriol. Rev. 32: 320–348.
Cuzin, F. et Jacob, F. 1964. Délétions chromosomiques et intégration d'un episome sexual F lac + chez Escherichia coli K 12.—C. R. Acad. Sci. (Paris) 258: 1350–1352.
Davis, B. R. and Ewing, W. H. 1966. The biochemical reactions of Citrobacter freundii.—N.C.D.C. Publ., National Communicable Disease Center, Atlanta, Ga.
Demerec, M., Adelberg, E. A., Clark, A. J. and Hartman, Ph. E. 1966. A proposal for a uniform nomenclature in bacterial genetics—Genetics Princeton 54: 61–76.
Edwards, P. R. and Ewing, W. H. 1962. Identification of Enterobacteriaceae. 2nd ed.—Burgess Publishing Company, Atlanta, Ga.
Ewing, W. H. 1968. Differentiation of Enterobacteriaceae by biochemical reactions.—N.C.D.C. Publ., National Communicable Disease Center, Atlanta Ga.
De Graaff, J., Barendsen, W. and Stouthamer, A. H. 1973a. Mapping of chlorate-resistant mutants of Citrobacter freundii by deletion and complementation analysis.—Mol. Gen. Genet. 121: 259–269.
de Graaff, J., Kreuning, P. C. and Van de Putte, P. 1973b. Host controlled restriction and modification of bacteriophage Mu and Mu-promoted chromosome mobilization in Citrobacter freundii.—Mol. Gen. Genet. 123: 283–288.
De Graaff, J. and Stouthamer, A. H. 1971. Citrobacter freundii mutants deficient in host specificity functions and their recipient ability for foreign deoxyribonucleic acid.—J. Gen. Microbiol. 67: 91–97.
De Graaff, J. and Stouthamer, A. H. 1972. Properties of a temperature sensitive plasmid in Citrobacter freundii.—Mol. Gen. Genet. 119: 259–266.
De Haan, P. G. and Gross, J. D. 1962. Transfer delay and chromosome withdrawal during conjugation in Escherichia coli.—Genet. Res. Camb. 3: 251–272.
Hayes, W. 1968. The genetics of bacteria and their viruses, 2nd ed.—Blackwell Scientific Publications, Edinburgh.
Kétyi, I. 1970. Isolation of Hfr derivative by the use of Shigella flexneri 4b-modified F factor. —Acta Microbiol. Acad. Sci. Hung. 17: 167–173.
Low, B. 1965. Low recombination frequency for markers very near the origin in conjugation in E. coli.—Genet. Res. Camb. 6: 469–473.
Machtiger, N. A. and O'Leary, W. M. 1973. Fatty acid compositions of paracolons: Arizona, Citrobacter and Providencia.—J. Bacteriol. 114: 80–85.
Matney, T. S. and Achenbach, N. E. 1962. A comment on the fertility of F2 donor types of Escherichia coli K 12.—Biochem. Biophys. Res. Commun. 9: 285–287.
Matsumoto, H. and Tazaki, T. 1971. Genetic recombination in Klebsiella pneumoniae. An approach to genetic linkage mapping.—Jap. J. Microbiol. 14: 129–141.
Matsumoto, H. and Tazaki, T. 1971. Genetic mapping of aro, pyr and pur markers in Klebsiella pneumoniae.—Jap. J. Microbiol. 15: 11–20.
Ou, J. T. and Anderson, T. F. 1970. Role of pili in bacterial conjugation.—J. Bacteriol. 102: 648–654.
Pittard, J. and Walker, E. M. 1967. Conjugation in Escherichia coli: recombination events in terminal regions of transferred deoxyribonucleic acid.—J. Bacteriol. 94: 1656–1663.
Sanderson, K. E. 1971. Genetic homology in the Enterobacteriaceae.—Advan. Genet. 16: 35–51.
Sanderson, K. E. 1972. Linkage map of Salmonella typhimurium, Edition IV.—Bacteriol. Rev. 36: 558–586.
Sanderson, K. E. and Demerec, M. 1965. The linkage map of Salmonella typhimurium.—Genetics Princeton 51: 897–913.
Sanderson, K. E., Ross, H., Ziegler, L. and Mäkelä, P. H. 1972. F+, Hfr, and F′ strains of Salmonella typhimurium and Salmonella abony.—Bacteriol. Rev. 36: 608–637.
Schneider, H. and Falkow, S. 1964. Characterization of an Hfr strain of Shigella flexneri.—J. Bacteriol. 88: 682–689.
Taylor, A. L. and Trotter, C. D. 1972. Linkage map of Escherichia coli K-12.—Bacteriol. Rev. 36: 504–524.
Zinder, N. D. 1960. Sexuality and mating in Salmonella.—Science 131: 924–926.
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de Graaff, J., Kreuning, P.C. & Stouthamer, A.H. Isolation and characterization of Hfr males in Citrobacter freundii . Antonie van Leeuwenhoek 40, 161–170 (1974). https://doi.org/10.1007/BF00394563
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DOI: https://doi.org/10.1007/BF00394563