Skip to main content
SpringerLink
Log in

A true microculture technique for human lymphocytes

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

A true microculture technique utilizing only 0.01 ml of whole blood is presented. The blood is cultured in Eppendorf plastic microtubes in 1 ml of medium. This technique is easier, faster, and more economical than conventional methods. It is suitable for situations in which interphase cells or a small number (≤50) of metaphase cells are required.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Evans, H. J.: A simple microculture technique for obtaining human chromosome preparations with some comments on DNA replication in sex chromosomes of the goat, cow and pig. Expt'l Cell Res. 38, 511 (1965)

    Google Scholar 

  • German, J.: Chromosomal breakage syndromes. Birth Defects: Original Article Series, Vol. V, 117 (1969)

    Google Scholar 

  • Heddle, J. A.: A rapid in vivo test for chromosomal damage. Mutat. Res. 18, 187 (1973)

    Google Scholar 

  • Khudr, G., Benirschke, K.: Cytogenetic micromethod on fetal blood. Gynec. Invest. 4, 172 (1973)

    Google Scholar 

  • Leeson, Thomas S., Leeson, C. Roland: Histology, W. B. Saunders Co. Publ, (Toronto) (1970)

    Google Scholar 

  • Moorhead, P. S., Nowell, P. C., Mellman, W. J., Battips, D. N., Hungerford, D. A.: Chromosome preparations of leukocytes cultured from human peripheral blood. Expt'l Cell Res. 20, 613 (1960)

    Google Scholar 

  • Schmid, W.: The micronucleus test. Mutat. Res. 31, 9 (1975)

    Google Scholar 

  • Turpin, R., Lejeune, J.: Human Afflications and Chromosomal Aberrations. pp. 392. First English Edition. Paris: Pergamon Press 1969

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, York University, M3J IP3, Downsview, Ontario, Canada

    Paul I. Countryman & John A. Heddle

  2. Department of Natural Science, York University, M3J IP3, Downsview, Ontario, Canada

    Paul I. Countryman & John A. Heddle

Authors
  1. Paul I. Countryman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John A. Heddle
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Countryman, P.I., Heddle, J.A. A true microculture technique for human lymphocytes. Hum Genet 35, 197–200 (1977). https://doi.org/10.1007/BF00393970

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00393970

Keywords

Search

Navigation