Abstract
Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood samples using the density gradient centrifugation. T-cells obtained using the protocol described here can be used for a variety of downstream analysis, including cellular, molecular, and functional assays.
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Acknowledgments
This work was supported by the Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant to N.K.V. and the Singapore Ministry of Education under its Singapore Ministry of Education (MOE) Academic Research Fund (AcRF) Tier 1 (2014-T1-001-141) and MOE-AcRF Tier 2 (MOE2017-T2-2-004). A.K. acknowledges Ph.D. fellowship provided by Lee Kong Chian School of Medicine, Nanyang Technological University Singapore.
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Kizhakeyil, A., Ong, S.T., Fazil, M.H.U.T., Chalasani, M.L.S., Prasannan, P., Verma, N.K. (2019). Isolation of Human Peripheral Blood T-Lymphocytes. In: Verma, N. (eds) T-Cell Motility. Methods in Molecular Biology, vol 1930. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9036-8_2
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DOI: https://doi.org/10.1007/978-1-4939-9036-8_2
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Print ISBN: 978-1-4939-9035-1
Online ISBN: 978-1-4939-9036-8
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