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Breakdown of phosphatidylinositol in soybean callus

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Abstract

We have investigated the breakdown of membrane-bound phosphatidylinositol (PI) in homogenates of soybean (Glycine max) callus. The breakdown of PI was stimulated by the detergent deoxycholate. At pH 7.0 and 1·gl-1 of deoxycholate the loss of PI was rapid and extensive: more than 80% was broken down within 10 min. The breakdown of PI was also stimulated by millimolar concentrations of Ca2+. The products of breakdown of added PI (purified from soybean callus) in this system were identified from their chromatographic mobilities as 1,2-diacylglycerol, myo-inositol 1-phosphate and myo-inositol 1:2-cyclic monophosphate.

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Abbreviations

DOC:

deoxycholate

EDTA:

ethylenedi-aminetetraacetic acid,-acetate

Pi:

Inorganic phosphate

PI:

phosphatidylinositol

PS:

phosphatidylserine

TLC:

thinlayer chromatography

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Author notes

  1. R. J. A. Connett

    Present address: Unit 154/155, Agricultural Genetics Company, Cambridge Science Park, CB4 4BH, Cambridge, UK

Authors and Affiliations

  1. Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK

    R. J. A. Connett & D. E. Hanke

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  1. R. J. A. Connett
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  2. D. E. Hanke
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Connett, R.J.A., Hanke, D.E. Breakdown of phosphatidylinositol in soybean callus. Planta 169, 216–221 (1986). https://doi.org/10.1007/BF00392317

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  • DOI: https://doi.org/10.1007/BF00392317

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