Abstract
A cell-free system prepared from developing seed of runner bean (Phaseolus coccineus L.) converted [14C]gibberellin A12-aldehyde to several products. Thirteen of these were identified by capillary gas chromatography-mass spectrometry as gibberellin A1 (GA1), GA4, GA5, GA6, GA15, GA17, GA19, GA20, GA24, GA37, GA38, GA44 and GA53-aldehyde, all giving mass spectra with 14C-isotope peaks. GA8 and GA28 were also identified but contained no 14C. All the [14C]GA12-aldehyde metabolites, except GA15, GA24 and GA53-aldehyde, are known endogenous GAs of P. coccineus.
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Abbreviations
- GAn :
-
gibberellin An
- GC-MS:
-
combined gas chromatography-mass spectrometry
- HPLC:
-
highperformance liquid chromatography
- MVA:
-
mevalonic acid
- S-2:
-
2000-g supernatant
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Turnbull, C.G.N., Crozier, A., Schwenen, L. et al. Conversion of [14C]gibberellin A12-aldehyde to C19- and C20-gibberellins in a cell-free system from immature seed of Phaseolus coccineus L.. Planta 165, 108–113 (1985). https://doi.org/10.1007/BF00392218
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DOI: https://doi.org/10.1007/BF00392218