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The induction of phenylpropanoid biosynthetic enzymes by ultraviolet light or fungal elicitor in cultured parsley cells is overriden by a heat-shock treatment

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Abstract

The normal (25° C) response of parsley (Petroselinum crispum Mill.) cell suspension cultures to ultraviolet (UV) light was suppressed by a simultaneous 37° C heat-shock treatment, as indicated by the loss of the inducibility of two enzymes of flavonoid biosynthesis, phenylalanine ammonia-lyase (EC 4.3.1.5) and chalcone synthase. The effects on enzyme activity and on enzyme synthesis in vitro and in vivo were similar, indicating that regulatory control is at an early step of gene expression, presumably transcription. When heat shock was given during the course of an ongoing UV induction, both enzyme synthesis and enzyme activities ceased rapidly. Likewise, the induction of phenylalanine ammonia-lyase by an elicitor from Phytophthora megasperma f. sp. glycinea was terminated upon transfer from 25° C to 37° C. Based on these and previously published data, it is concluded that stress responses in this system are preferentially expressed in the order of heat shock, fungal elicitor and UV light.

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Abbreviations

CHS:

chalcone synthase

HSP:

heat-shock protein

PAL:

phenylalanine ammonia-lyase

UV:

ultraviolet

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  1. Michael H. Walter

    Present address: Institut für Pflanzenphysiologie-260-, Universität Hohenheim, Emil Wolff-Strasse 25, D-7000, Stuttgart 70, Federal Republic of Germany

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  1. Institut für Biologie II der Universität, Schänzlestrasse 1, D-7800, Freiburg i.Br., Federal Republic of Germany

    Michael H. Walter

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Walter, M.H. The induction of phenylpropanoid biosynthetic enzymes by ultraviolet light or fungal elicitor in cultured parsley cells is overriden by a heat-shock treatment. Planta 177, 1–8 (1989). https://doi.org/10.1007/BF00392148

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  • DOI: https://doi.org/10.1007/BF00392148

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