Abstract
The crude lysosomal fraction of corn seedling root tips contains an arylsulphatase (E.C. 3.1.6.1) which hydrolysed p-nitrophenyl sulphate at pH 8.0 but had no activity towards p-nitrocatechol sulphate. The Km value for p-nitrophenyl sulphate was 1.24 mM. The hydrolysis of p-nitrophenyl sulphate was linear up to 2 h and the rate was proportional to the amount of enzyme added. The enzyme was strongly inhibited by cyanide, fluoride and phosphate ions and did not resemble the arylsulphatases of bacterial and animal origin.
Similar content being viewed by others
References
Baum, H., Dodgson, K.S.: Differentiation between myrosulphatase and the arylsulphatases. Nature 179, 312–313 (1957)
Dodgson, K.S., Spencer, B.: In: Methods of biochemical analysis. vol IV, pp. 211–255 Glick, D., ed., New York, London: Interscience Publishers 1957
Farooqui, A.A., Bachhawat, B.K.: Regional distribution, age dependent variation and species differences of brain arylsulphatases. J. Neurochem. 18, 635–646 (1971)
Farooqui, A.A.: Purification and properties of human placenta arylsulphatase A. Arch. Internat. Physiol. Biochim. (In press) (1976a)
Farooqui, A.A.: Desulphation of hexosamine sulphate by arylsulphatase B. Experientia, In press (1976b)
Farooqui, A.A., Roy, A.B.: The preparation of sulphatases B1α and B1β. Biochim. Biophys. Acta. In press (1976)
Heftmann, E.: Lysosomes in tomatoes. Cytobios 3, 129–136 (1971)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.L.: Protein measurement with Folin reagent. J. biol. Chem. 193, 265–275 (1951)
Mattile, P.: Lysosomes of root tip cells in corn seedlings. Planta 79, 181–196 (1968)
Mehl, E., Jatzkewitz, H.: Cerebroside 3-sulphate as a physiological substrate of arylsulphatase A. Biochim. Biophys. Acta 151, 619–627 (1968)
Pitt, D., Galpin, M.: Isolation and properties of lysosomes from dark-grown potato shoots. Planta 109, 233–258 (1973)
Poux, N.: Ultrastructural localization of arylsulphatase activity in plant meristematic cells. J. Histochem. Cytochem. 14, 932–933 (1966)
Roy, A.B., Trudinger, P.: In: The biochemistry of inorganic compounds of sulphur. pp. 133–189, Cambridge; Cambridge University Press, 1970
Roy, A.B.: The hydrolysis of sulphate esters. In: The enzymes vol V, pp. 1–19 (Boyer, p ed) New York-London: Academic Press, 1971
Schiff, J.A., Hodson, R.C.: The metabolism of sulphate. Ann. Rev. Plant Physiol. 24, 381–414 (1973)
Semadeni, E.G.: Enzymatische Charakterisierung der Lysosomenäquivalente (Sphärosomen) von Maiskeimlingen. Planta (Berl.) 72, 91–118 (1967)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Farooqui, A.A., Samiullah & Afridi, M.M.R.K. Arylsulphatase activity of corn (Zea mays L.) seedling roots. Planta 133, 157–160 (1977). https://doi.org/10.1007/BF00391914
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00391914