Summary
Human lymphocytes and lymphoid cell lines were analyzed for the presence of complex forms of mitochondrial DNA (mtDNA) by electron microscopy. A high frequency (9%–14.5%) of catenated dimers, circular dimers, or oligomers were found in samples from Epstein-Barr-virus-(EBV) transformed lymphoblastoid cell lines. These complex forms of mtDNA were present in much lower frequencies in lymphocytes isolated from donor blood (1.3%–4.6%). Similar low frequencies were found with primary fibroblasts (1.1%) or freshly isolated monkey liver cells (2.1%). Samples from cultures of Burkitt lymphoma (BL) cell lines of EBV_positive or-negative origin contained intermediate (5%–7%) frequencies of complex forms of mtDNA.
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Supported by grants from the Fogh-Nielsen Foundation, Aarhus Universitets Forskningsfond, Fonden til Laegevidenskabens Fremme, and by Grants 11-2231 and 12-2587 from the Danish Natural Science and Medical Research Councils
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Christiansen, G., Christiansen, C. & Zeuthen, J. Complex forms of mitochondrial DNA in human B cells transformed by Epstein-Barr virus (EBV). J Cancer Res Clin Oncol 105, 13–19 (1983). https://doi.org/10.1007/BF00391825
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DOI: https://doi.org/10.1007/BF00391825