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Two Extramitochondrial Circular DNA Species in the Petite Negative Yeast Schizosaccharomyces pombe: Relative abundance and size determination by electron microscopy

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In this paper we present the electron microscopic analysis of two distinct extramitochondrial circular DNA species in the fission yeast Schizosaccharomyces pombe (S. pombe). Both DNA species can be isolated from mitochondrial fractions, but disappear after DNase treatment of mitochondria, demonstrating their extramitochondrial location. The size of these molecular species is 3.08 ± 0.18 μm and 2.00 ± 0.09 μm (standard deviation). They are present in a ratio of approximately 9:1 in the DNA preparations analyzed.

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Authors and Affiliations

  1. Istituto Internazionale di Genetica e Biofisica, Consiglio Nazionale delle Ricerche, Via G. Marconi 10, 80125 Napoli, Italy

    F. Manna, L. Del Giudice & W. H. Schreil

  2. Institut für Genetik und Mikrobiologie der Universität München, Maria Ward Straße 1a, 8000, München 19, Germany

    K. Wolf

Authors
  1. F. Manna
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  2. L. Del Giudice
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  3. W. H. Schreil
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  4. K. Wolf
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Manna, F., Del Giudice, L., Schreil, W.H. et al. Two Extramitochondrial Circular DNA Species in the Petite Negative Yeast Schizosaccharomyces pombe: Relative abundance and size determination by electron microscopy. Curr Genet 5, 187–189 (1982). https://doi.org/10.1007/BF00391804

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  • DOI: https://doi.org/10.1007/BF00391804

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