Abstract
Several lines of evidence indicate that the conversion of 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene by microsomal membranes from carnation flowers is attributable to hydroperoxides generated by membrane-associated lipoxygenase (EC 1.13.11.12). As the flowers senesce, the capability of isolated microsomal membranes to convert ACC to ethylene changes. This pattern of change, which is distinguishable from that for senescing intact flowers, shows a close temporal correlation with levels of lipid hydroperoxides formed by lipoxygenase in the same membranes. Specific inhibitors of lipoxygenase curtail the formation of lipid hydroperoxides and the production of ethylene from ACC to much the same extent, whereas treatment of microsomes with phospholipase A2, which generates fatty-acid substrates for lipoxygenase, enhances the production of hydroperoxides as well as the conversion of ACC to ethylene. Lipoxygenase-generated lipid hydroperoxides mediate the conversion of ACC to ethylene in a strictly chemical system and also enhance ethylene production by microsomal membranes. The data collectively indicate that the in-vitro conversion ACC to ethylene by microsomal membranes of carnation flowers is not reflective of the reaction mediated by the native in-situ ethylene-forming enzyme.
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Abbreviations
- ACC:
-
1-aminocyclopropane-1-carboxylic acid
- EDTA:
-
ethylenediaminetetraacetic acid
References
Bousquet, J.F., Thimann, K.V. (1984) Lipid peroxidation forms ethylene from 1-aminocyclopropane-1-carboxylic acid and may operate in leaf senescence. Proc. Natl. Acad. Sci. USA 81, 1724–1727
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254
Buege, J.A., Aust, S.D. (1978) Microsomal lipid peroxidation. Methods Enzymol. 52, 302–309
Guy, M., Kende, H. (1984) Conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene by isolated vacuoles of Pisum sativum L. Planta 160, 281–287
Hoffman, N.E., Yang, S.F., Ichihara, A., Sakamura, S. (1982) Stereopecific conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene by plant tissues. Conversion of stereoisomeres of 1-amino-2-ethylcyclopropane-1-carboxylic acid to 1-butene. Plant Physiol. 70, 195–199
Konze, J.R., Kende, H. (1979) Ethylene formation from 1-aminocyclopropane-1-carboxylic acid in homogenates of etiolated pea seedlings. Planta 146, 293–301
Legge, R.L., Thompson, J.E. (1983) Involvement of hydroper-oxides and an ACC-derived free radical in the formation of ethylene. Phytochemistry 22, 2161–2166
Lynch, D.V., Thompson, J.E. (1984) Lipoxygenase-mediated production of superoxide anion during plant senescence. FEBS Lett. 173, 251–254
Mayak, S., Legge, R.L., Thompson, J.E. (1981) Ethylene formation from 1-aminocyclopropane-1-carboxylic acid by microsomal membranes from senescing carnation flowers. Planta 153, 49–55
Mayak, S., Legge, R.L., Thompson, J.E. (1983) Superoxide radical production by microsomal membranes from senescing carnation flowers: an effect on membrane fluidity. Phytochemistry 22, 1375–1380
McKeon, T.A., Yang, S.F. (1984) A comparison of the conversion of 1-amino-2-ethylcyclopropane-1-carboxylic acid stereoisomers to 1-butene by pea epicotyls and by a cell free system. Planta 160, 84–87
St. Angelo, A.J., Ory, R.L. (1984) Lipoxygenase inhibition by naturally occurring monoenoic fatty acids. Lipids 19, 34–37
Venis, M.A. (1984) Cell-free ethylene-forming systems lack stereochemical fidelity. Planta 162, 85–88
Wallach, D.P., Brown, V.R. (1981) A novel preparation of human platelet lipoxygenase. Characteristics and inhibition by a variety of phenyl hydrazones and comparisons with other lipoxygenases. Biochim. Biophys. Acta 663, 361–372
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Lynch, D.V., Sridhara, S. & Thompson, J.E. Lipoxygenase-generated hydroperoxides account for the nonphysiological features of ethylene formation from 1-aminocyclopropane-1-carboxylic acid by microsomal membranes of carnations. Planta 164, 121–125 (1985). https://doi.org/10.1007/BF00391036
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DOI: https://doi.org/10.1007/BF00391036