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Separation and localization of two classes of auxin binding sites in corn coleoptile membranes

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Summary

Further evidence is presented for the discrete nature of the two classes of high affinity auxin binding sites in corn (Zea mays L.) coleoptile membranes, site 1 and site 2. Fractions can be obtained by differential centrifugation that exhibit binding kinetics characteristic of site 2, but not site 1. Membrane preparations containing both binding sites may be resolved on sucrose gradients into a light and a heavy band, whose binding kinetics and analogue binding specificities correspond to those deduced for site 1 and site 2 respectively in unfractionated membranes. Evidence from enzymic and chemical assays and from electron microscopy suggests that site 2, the auxin-specific binding site, is located in fractions enriched in plasma membrane, whereas site 1 is associated with Golgi membranes and/or endoplasmic reticulum.

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Abbreviations

NAA:

1-naphthylacetic acid

IAA:

3-indolylacetic acid

TIBA:

2,3,5-triiodobenzoic acid

SDH:

succinic dehydrogenase

IDPase:

inosine diphosphatase

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Author notes

  1. Susan Batt

    Present address: Department of Biology, University of Utah, S4112, Salt Lake City, Utah, USA

Authors and Affiliations

  1. Department of Botany, University of Glasgow, G12 8QQ, Glasgow, UK

    Susan Batt

  2. Woodstock Laboratory, Sittingbourne Research Centre, ME9 8AG, Sittingbourne, Kent, UK

    Michael A. Venis

Authors
  1. Susan Batt
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  2. Michael A. Venis
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Batt, S., Venis, M.A. Separation and localization of two classes of auxin binding sites in corn coleoptile membranes. Planta 130, 15–21 (1976). https://doi.org/10.1007/BF00390839

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  • DOI: https://doi.org/10.1007/BF00390839

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