Summary
Cell division was synchronised in 4-litre batch cultures of Acer pseudoplatanus L. by starvation and regrowth. Up to five consecutive cell cycles were observed in each culture. Mitosis and cytokinesis were synchronised within 0.2 cell cycles. Accumulation of extractable DNA was discontinuous and separate from cytokinesis. Correction for the degree of synchrony in the population gave: G1=13–37 h, S=15 h, G2=14–19 h and M=0.9–1.3 h. Thymidine kinase activity and [14C]thymidine incorporation were highest during S-phase. A peak of activity of aspartate transcarbamoylase occurred during G2. Peaks in succinate dehydrogenase activity and respiration rate were observed at the initiation of DNA synthesis and just prior to mitosis. The activity of glucose-6-phosphate dehydrogenase doubled in one step during the cell cycle. Total RNA and protein accumulated continuously through the cell cycle; the final rate being twice that observed initially.
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References
Bonner, W. J.: Succinic dehydrogenase. In: Methods in enzymology, vol. 1, p. 722–729, Kaplan, C., ed. New York-London: Academic Press 1953
Cox, B. J., Turnock, G., Street, H. E.: Studies on the growth in culture of plant cells. XV. Uptake and utilization of uridine during the growth of Acer pseudoplatanus L. cells in suspension culture. J. exp. Bot. 24, 159–174 (1973)
Dewey, W. L., Miller, H. H., Nagasawa, H.: Interactions between S and G1 cells. Exp. Cell Res. 77, 73–78 (1973)
Donachie, W., Masters, N.: Temporal control of gene expression in bacteria. In: The cell cycle: gene-enzyme interactions, p. 37–76, Padilla, G., Whitson, G., Cameron, I. L., eds. New York-London: Academic Press 1969
Eriksson, T.: Partial synchronization of cell division in suspension cultures of Haplopappus gracilis. Physiologia Plantarum (Cph.) 19, 900–910 (1966)
Evans, G. M., Rees, H.: Mitotic cycles in dicotyledons and monocotyledons. Nature (Lond.) 233, 350–351 (1971)
Fowler, M. W.: Studies on the growth in culture of plant cells. XIV. Carbohydrate oxidation during the growth of Acer pseudoplatanus L. cells in suspension culture. J. exp. Bot. 22, 715–724 (1971)
Gould, A. R., Bayliss, M. W., Street, H. E.: Studies on the growth in culture of plant cells. XVII. Analysis of the cell cycle of asynchronously dividing Acer pseudoplatanus L. cells in suspension culture. J. exp. Bot. (in press) (1974)
Henshaw, G. G., Jha, K. K., Mehta, A. R., Shakeshaft, O. J., Street, H. E.: Studies on the growth in culture of plant cells. I. Growth patterns in batch propagated suspension cultures. J. exp. Bot. 17, 362–377 (1966)
Hiraga, S., Igarashi, K., Yura, T.: A deoxythymidine kinase-deficient mutant of Escherichia coli. 1. Isolation and some properties. Biochim. biophys. Acta (Amst.) 145, 41–51 (1967)
Holdgate, D. P., Goodwin, T. W.: Quantitative extraction and estimation of plant nucleic acids. Phytochemistry 4, 831–843 (1965)
Hotta, Y., Stern, H. S.: Inducibility of thymidine kinase by thymidine as a function of interphase stage. J. Cell Biol. 25, 99–108 (1965)
Jouanneau, J. P.: Contrôle par les cytokinines de la synchronisation des mitoses dans les cellules de tabac. Exp. Cell Res. 67, 329–337 (1971)
King, P. J.: The continuous culture of plant cells. Ph. D. thesis, University of Leicester (1973)
King, P. J., Mansfield, K. J., Street, H. E.: Control of growth and metabolism in cultured plant cells. Canad. J. Bot. 51, 1807–1823 (1973)
King, P. J., Street, H. E.: Growth patterns in cell cultures. In: Plant tissue and cell culture, p. 269–337, Street, H. E., ed. Oxford: Blackwells 1973
Kovacs, C. J., Van't Hof, J.: Synchronization of a proliferative population in a cultured plant tissue. J. Cell Biol. 47, 536–539 (1970)
Layne, E.: Spectrophotometric and turbidimetric methods for measuring proteins. In: Methods in enzymology, vol. 3, p. 447–454, Kaplan, C., ed. New York-London: Academic Press 1957
Long, C.: ed., Biochemists' handbook, p. 995. London: Spon 1961
Mitchison, J. M.: The biology of the cell cycle. Cambridge: University Press 1971
Neumann, J., Jones, M. E.: Aspartic transcarbamoylase from lettuce seedlingscase of end-product inhibition. Nature (Lond.) 195, 709–710 (1962)
Okamura, S., Miyasaka, K., Nishi, A.: Synchronisation of carrot cell culture by starvation and cold treatment. Exp. Cell Res. 78, 467–470 (1973)
Padilla, G., Whitson, G., Cameron, I. L.: Eds. The cell cycle, gene—enzyme interactions. New York-London: Academic Press 1969
Shepherdson, M., Pardee, A. B.: Production and crystallization of aspartate transcarbamoylase. J. biol. Chem. 235, 3233–3237 (1960)
Short, K. C., Brown, E. G., Street, H. E.: Studies on the growth in culture of plant cells. VI. Nucleic acid metabolism of Acer pseudoplatanus L. cell suspensions. J. exp. Bot. 20, 579–590 (1969)
Stuart, R., Street, H. E.: Studies on the growth in culture of plant cells. IV. The initiation of division in suspensions of stationary-phase cells of Acer pseudoplatanus L. J. exp. Bot. 20, 556–571 (1969)
Van't Hof, J., Kovacs, C. J.: Mitotic cycle regulation in the meristem of cultured roots: the principal control point hypothesis. Adv. Exp. Med. 18, 15–30 (1972)
Van't Hof, J., Sparrow, A. H.: A relationship between DNA content, nuclear volume and minimum mitotic cycle time. Proc. nat. Acad. Sci. (Wash.) 49, 897–902 (1963)
Vassef, A. A., Flora, J. B., Weeks, J. G., Bibbs, B. S., Schmidt, R.: The effects of enzyme synthesis and stability and of deoxyribonucleic acid replication on the cellular levels of aspartate transcarbamoylase during the cell cycle of Eukaryote Chlorella. J. biol. Chem. 248, 1976–1985 (1973)
Wilson, S. B., King, P. J., Street, H. E.: Studies on the growth in culture of plant cells. XII. A versatile system for the large scale batch or continuous culture of plant cell suspensions. J. exp. Bot. 22, 177–207 (1971)
Yeoman, M. M., Aitchison, P. A.: Changes in enzyme activities during the division cycle of cultured plant cells. In: The cell cycle in development and differentiation, p. 185–201, Balls, M., Billett, F. S., eds. Cambridge: University Press 1973
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King, P.J., Cox, B.J., Fowler, M.W. et al. Metabolic events in synchronised cell cultures of Acer pseudoplatanus L.. Planta 117, 109–122 (1974). https://doi.org/10.1007/BF00390793
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DOI: https://doi.org/10.1007/BF00390793